核酸分析仪校准结果不确定度的评定

Evaluation of Uncertainty of Nucleic Acid Analyzer Calibration Results

核酸分析仪是基于凝胶电泳技术原理,将不同大小的双链核酸片段进行分离,在荧光染料存在的条件下,荧光染料嵌入双链核酸中发出荧光,对荧光信号进行检测分析,可根据各核酸片段的电泳时间和荧光信号值进行定性、定量分析[1]。可用于测量DNA,RNA纯度、浓度,测量蛋白质浓度,也可用于一般物质分析中的吸光度检测[2-3]。本文对核酸分析仪不确定度的来源进行了分析,并对各不确定度分量进行计算,给出了长度示值误差、浓度示值误差的扩展不确定度。

Nucleic acid analyzers is based on the principle of gel electrophoresis technology,the different sizes of double-stranded DNA fragments were separated,in the presence of fluorescent dyes,fluorescent dyes embedded fluoresce in double-stranded nucleic acid,the fluorescent signal was analyzed,according to the time of segments of nucleic acid electrophoresis and fluorescence signal value,qualitative and quantitative analysis were discussed.It can be used to measure DNA,RNA purity,concentration,protein concentration,also can be used for general material analysis in the absorbance detection.The source of uncertainty of nucleic acid analyzer was analyzed,and the uncertainty components were calculated,and the extended uncertainty of length indication error and concentration indication error was given.