益生菌冲剂的体外模拟消化及抗菌活性研究

Study on Simulated Digestion in Vitro and Antibacterial Activity of Probiotics Granules

为了解益生菌冲剂中益生菌在肠道中的活性和抗菌性,本文测定了市面上3个不同品牌的益生菌冲剂(A、B、C)中所含益生菌经体外胃肠道模型消化后的最终存活率和对大肠杆菌的抗菌活性。结果表明,经体外模拟消化后3个产品中只有A产品有菌落生长,存活率为0.00004%,3种益生菌补充剂对胃肠道消化耐受性能差异较大;3种产品中的益生菌都对大肠杆菌有一定的抑制能力,且抑菌活性的大小为C>A>B。本研究结果可为开发更好的益生菌产品提供参考。

In order to understand the stability of probiotics of probiotic granule products in the digestive tract and their antibacterial activity,the survival ability of probiotics in 3 commercial probiotic granule products(designated as A,B and C)was analyzed using an in vitro gastrointestinal model.Antibacterial activity of these three products against Escherichia coli was also examined.After exposed to the in vitro gastrointestinal model,only product A had colonial growth,and the survival rate was 0.00004%.It was found that the three probiotic granule products had great differences in gastrointestinal digestive tolerance.The probiotics in the three products all showed certain inhibitory capacity against E.coli,and the antibacterial activity was C>A>B.The results can provide a valuable reference for future development of probiotic products.