拟南芥N末端乙酰转移酶Naa50参与调控根细胞有丝分裂

Arabidopsis N-terminal acetyltransferase Naa50 is involved in regulation of root cell mitosis

N末端乙酰转移酶Naa50在调控拟南芥生长发育方面至关重要。为探究Naa50是否通过调控细胞有丝分裂来影响植物生长发育,将naa 50-1突变体与H2B-YFP株系杂交,利用H2B-YFP标示细胞分裂过程中染色体的分离行为。结果表明,与野生型相比,拟南芥naa 50-1突变体根尖分生区细胞有丝分裂指数下降,染色体畸变率明显增加,微核率上升,且处在分裂中期的细胞比例增加。将naa 50-1突变体与CYCB1-CUS株系杂交,检测根细胞中CYCB1蛋白表达,发现naa 50-1突变体中CYCB1蛋白表达量明显增加。PI染色分析表明,naa 50-1突变体根部存在部分死细胞。以上结果表明,在细胞有丝分裂过程中,Naa50促进遗传物质平均分配到两个子细胞中,促进细胞从分裂中期向后期转换;Naa50通过调控细胞分裂进而参与植物生长发育的调控。

N-terminal acetyltransferase Naa50 plays an important role in regulating growth and development in Arabidopsis.In order to explore whether Naa50 affects plant growth and development by regulating cell mitosis,the naa 50-1 mutant was crossed with H2B-YFP line,and the segregation behavior of chromosomes during cell division was detected by H2B-YFP.The results showed that compared with the wild-type,the cell mitotic index in root meristem region of naa 50-1 mutant decreased,chromosome aberration rate and micronucleus rate increased,and so as the proportion of cells in metaphase increased.The expression of CYCB1 protein in root cells was detected by crossing of naa 50-1 mutant with CYCB1-CUS,and it was found that the expression of CYCB1 protein in naa 50-1 mutant was increased.PI staining analysis showed that there were several dead cells in the root of naa 50-1 mutant.These results indicated that Naa50 promotes the equal distribution of genetic material between two daughter cells during mitosis and the transition from metaphase to anaphase.Overall,Naa50 is involved in the regulation of plant growth and development by regulating cell division.