茶叶茶氨酸与酸枣仁水提物的改善睡眠作用的研究

Study on the Effect of Theanine in Tea and Jujube Seed Water Extract on Improving Sleep

目的:研究茶叶茶氨酸、酸枣仁水提物及其复合物(1∶1)对改善睡眠的作用。方法:利用戊四唑(Pentylenetetrazol,PTZ)溶液诱导斑马鱼并建立失眠模型后,分别给予不同浓度的茶叶茶氨酸、酸枣仁水提物和酸枣仁茶叶茶氨酸复合物(1∶1)溶液,采用行为分析仪测定给药后斑马鱼的总运动距离,以总运动距离评价茶叶茶氨酸、酸枣仁水提物和酸枣仁茶叶茶氨酸复合物对PTZ诱导的斑马鱼失眠症的睡眠改善作用。结果:62.5μg·mL^(-1)、125.0μg·mL^(-1)和250.0μg·mL^(-1)的酸枣仁水提物对PTZ诱导的失眠斑马鱼的睡眠改善效果分别为40%、89%和109%;在125.0μg·mL^(-1)浓度条件下,茶叶茶氨酸对失眠斑马鱼具有突出的睡眠改善作用,达到了63%,同时,在62.5μg·mL^(-1)和250.0μg·mL^(-1)浓度条件下,茶叶茶氨酸也表现出了较好的睡眠改善作用,分别为30%和29%;此外,250.0μg·mL^(-1)和500.0μg·mL^(-1)的酸枣仁茶叶茶氨酸复合物对失眠斑马鱼也具有显著的睡眠改善作用,分别为80%和113%。结论:茶叶茶氨酸、酸枣仁水提物和酸枣仁茶叶茶氨酸复合物都具有一定改善睡眠的作用,可作为睡眠改善辅助治疗的功效成分应用于普通食品、保健食品和药品开发。

Objective:The effects of theanine from tea leaves,water extracts of wild jujube kernel and their compounds(1∶1)on sleep were studied.Method:After zebrafish insomnia model established by pentylenetetrazol(PTZ)induced,a variety of concentration of theanine,water extracts of wild jujube kernel and their complex were given to treatment group respectively.The total movement distance of zebrafish after administration was measured by behavior analyzer,and the sleep improvement effect of tea theanine,water extract of wild jujube kernel and tea theanine complex of wild jujube kernel on PTZ induced insomnia of zebrafish was evaluated by total movement distance.Result:62.5μg·mL^(-1),125.0μg·mL^(-1) and 250.0μg·mL^(-1) of water extracts of wild jujube kernel all showed a remarkable effect of sleep improvement on PTZ-induced insomnia zebrafish(40%、89% and 109%,respectively),and 125.0μg·mL^(-1) of theanine solution indicated a prominent enhancement function on sleep quality(63%).Meanwhile,62.5μg·mL^(-1) and 250.0μg·mL^(-1) of theanine solution demonstrated a better effect on improving sleep quality(30% and 29%).In addition,250.0μg·mL^(-1) and 500.0μg·mL^(-1) of complex solution were all proved to have a noteworthy sleep improvement effect(80% and 113%,respectively).Conclusion:To a certain extent,green tea-derived theanine,water extract of wild jujube kernel and complex of tea theanine and wild jujube kernel all have the effect of improving sleep,and can be used as the effective ingredient of sleep improvement auxiliary treatment in the development of general food,health food and medicine.