不同絮凝剂对光合细菌采收的影响

Effect on photosynthetic bacteria collection by different flocculants

相对于高速离心分离,采用絮凝技术采收光合细菌,具有耗能少、成本低、操作简单等特点。因此,本试验旨在研究不同质量浓度的硫酸铝、聚合氯化铝、硫酸亚铁、氯化铁及酵母细胞壁絮凝采收光合细菌的效果。结果显示:5种絮凝剂均能高效回收光合细菌,氯化铁采收光合细菌的最适质量浓度为1000 mg/L,回收率高达(98.11±0.24)%;硫酸铝的最适质量浓度为1000 mg/L,回收率为(94.12±0.13)%;硫酸亚铁的最适质量浓度为1500 mg/L,回收率为(90.56±0.16)%;酵母细胞壁的最适质量浓度为80 mg/L,回收率为(77.65±0.33)%;聚合氯化铝的最适质量浓度为2000 mg/L,回收率为(70.83±0.44)%。酵母细胞壁用量低、无毒害,更适合用于采收光合细菌;综合考虑,酵母细胞壁添加浓度以80 mg/L最宜,沉降浓缩时间为1 h。

Compared with high-speed centrifugal separation,the use of flocculation technology has the advantages of less energy consumption,low cost and simple operation,which provides a reference for the large-scale harvest of photosynthetic bacteria. Five flocculants,such as Al(SO),PAC,FeSO,Fe Cland yeast cell wall,were used to recover photosynthetic bacteria biomass. The results showed that all five flocculants could efficiently recover photosynthetic bacteria biomass. The optimum concentration of ferric chloride to collect photosynthetic bacteria was 1000 mg/L,and the recovery rate of photosynthetic bacteria biomass could reach as high as(98.11±0.24)%;The optimum concentration of aluminum sulfate was 1000 mg/L,which can bring a recovery rate of(94.12±0.13)%;The optimum concentration of ferrous sulfate was 1500 mg/L,and the recovery rate was(84.20 ± 0.02)%;The optimum concentration of yeast cell wall is 80 mg/L,and the recovery rate was(77.65±0.33)%;The optimum concentration of poly-aluminium chloride was 2000 mg/L,and the recovery rate was(70.83±0.44)%.Yeast cell wall was more suitable for collecting photosynthetic bacteria because of its low dosage and non-toxic. By comprehensive consideration,the added concentration of yeast cell wall should be 80 mg/L and the settling time should be 1 h.