应用LIS大数据建立佛山地区儿童血清睾酮和雌二醇参考区间

The reference interval of serum testosterone and estradiol in childrenin Foshan area was established by using LIS big data

目的应用实验室信息系统(LIS)大数据建立佛山地区儿童血清睾酮和雌二醇间接参考区间。方法收集2017年1月至2022年3月该院检验科LIS中8364例患者的睾酮和雌二醇检测值,筛选后最终纳入5228例患者的检测值进行后续研究。受检者年龄在0~18岁,按儿童的生长发育的七个阶段同时结合临床分为5个年龄组,婴儿时期(A组,0~1岁)、幼儿时期(B组,>1~3岁)、学龄前期(C组,>3~6岁)、学龄期(D组,>6~12岁)、青春期(E组,>12~18岁),结合R语言编程,线性拟合,建立间接参考区间。结果睾酮与年龄、性别有关(P<0.05),雌二醇与年龄、性别无关(P>0.05)。利用LIS存储数据建立了0~18岁佛山地区儿童各年龄段男童、女童睾酮和雌二醇的参考区间。结论应用LIS大数据建立睾酮和雌二醇的参考区间经济、简便、可行,可作为实验室建立参考区间的一种可行性选择,并可指导临床对儿童性早熟的诊断。

Objective To establish the indirect reference interval of serum testosterone and estradiol in children in Foshan area by applying the big data of laboratory information system(LIS).Methods From January 2017 to March 2022,the testosterone and estradiol detection data of 8364 patients with LIS in the clinical laboratory of the hospital were collected.After screening,the data of 5228 patients were finally included in the follow-up study.Subjects aged 0-18 years old were divided into five age group,infants(group A,0-1 years old)and young children(group B,>1-3 years old),preschool(group C,>3-6 years old),school-age(group D,>6-12 years old),adolescent(group E>12-18 years old)according to children′s growth and development of seven stages at the same time combined with clinical,the indirect reference interval was established by linear fitting combined with R language programming.Results Testosterone correlated with age and sex(P<0.05),estradiol was not correlated with age and sex(P>0.05).The reference intervals of testosterone and estradiol in male and female children aged 0-18 years in Foshan area were established based on LIS data.Conclusion It is economical,simple and feasible to establish the reference interval of testosterone and estradiol in LIS big data,which could be used as a feasible choice to establish the reference interval in laboratory,and can guide the clinical diagnosis of precocious puberty in children.