流式细胞术在神经细胞研究中的应用进展

Advances in the Application of Flow Cytometry in Nerve Cells Research

流式细胞术(flow cytometry,FCM)是一种对液流中排成单列的细胞或生物微粒逐个进行快速定量分析和分选的技术。近年来荧光驱动的细胞分选(fluorescence-activatied cell sorting,FACS)广泛应用于神经细胞研究领域中。该文将流式细胞分选与其他分选方法进行了对比,并评述了流式细胞术在神经细胞中的研究应用,总结了流式细胞术在神经细胞研究中的技术难点及解决途径,以期通过流式新技术更好地寻找针对神经系统发育相关性疾病的治疗思路和线索。

FCM (flow cytometry) is a rapid quantitative analysis and sorting technology of cells or biological particles in a liquid stream.FACS (fluorescence-activatied cell sorting) has been widely applied in the field of neuron cell research during the recent years.This paper compares FACS with other three sorting methods,and reviews the application of FCM in neuron cell research.At last,this article discusses the technical difficulties and solutions of FCM,expecting to look for the therapy clue of neuron system disease with new technology of flow cytometry.