高原移居与返回平原青年人群血液生化及细胞因子指标分析

Analysis of blood biochemical and cytokine indexes in young people immigrating to the plateau and returning to the plain

目的探讨高原移居青年人群与脱离高原返回平原和居住平原青年人群之间全血细胞、血清生化指标及炎症指标的差异。方法分别抽样纳入移居高原6个月至1年的青年人554例,以及同期脱离高原返回平原2个月左右的青年人536例和居住平原的青年人562例为研究对象,分为高原组、返回平原组和平原组;采集各组一般资料、血压、心率、血氧饱和度,检测其血常规、血糖、血脂、尿酸、肝肾功能指标,采用多重微球流式免疫荧光发光法和酶联免疫吸附试验检测血清多项细胞因子。结果与平原组、返回平原组相比,高原组舒张压、心率较高,血氧饱和度较低,外周血中红细胞、血红蛋白、白细胞、中性粒细胞水平较高,血清总胆红素、直接胆红素、天冬氨酸氨基转移酶、乳酸脱氢酶水平较高,血糖较低,差异均有统计学意义(P<0.05);高原组、返回平原组外周血中血小板计数较平原组低(P<0.05);与平原组相比,高原组血清尿酸、丙氨酸氨基转移酶水平较高(P<0.05);而返回平原组血清尿酸、丙氨酸氨基转移酶水平较高原组高(P<0.05)。与平原组、返回平原组相比,高原组血清白细胞介素(IL)-6、IL-8、IL-17A水平较高(P<0.05);返回平原组IL-6、IL-8水平仍较平原组高(P<0.05)。结论高原移居6个月至1年的青年人群多项血液指标及炎症细胞因子水平发生改变,脱离高原返回平原2个月左右的青年人群仍有部分指标未能恢复至居住平原的青年人群水平,表明高原缺氧可较长时间地影响机体代谢功能和诱导炎症反应。

Objective To explore the differences of whole blood cells,serum biochemical indexes and inflammatory indexes between the young people immigrating to the plateau,and those separating from the plateau to return to the plain and those living in the plain.Methods A total of 554 young people immigrating to the plateau for 6 months to 1 year,536 young people separating from the plateau to return to the plain for about 2 months and 562 young people from living in the plain were respectively sampled as the research subjects.They were divided into the plateau group,returning to plain group and plain group.The general data,blood pressure,heart rate and blood oxygen saturation of each group were collected,and the blood routine,blood glucose,blood lipids,uric acid,liver and kidney function indicators were detected.The multiple microsphere flow immunofluorescence assay and enzyme linked immunosorbent assay were used to detect the multiple serum cytokines indexes.Results Compared with the plain group and returning to plain group,the diastolic blood pressure,and heart rate in the plateau group were higher,the blood oxygen saturation was lower,peripheral red blood cells,hemoglobin,white blood cells and neutrophile granulocytes levels were higher,serum total bilirubin,direct bilirubin,aspartate aminotransferase and lactate dehydrogenase were higher,blood glucose was lower,and differences were statistically significant(P<0.05);the peripheral blood platelets in the plateau group and the returning to plain group were lower than those in the plain group(P<0.05);compared with the plain group,the serum uric acid and alanine-transaminases in the plateau group were higher,which in the returning to plain group were higher than those in the plateau group.Compared with the plain group and returning to plain group,the serum interleukin(IL)-6,IL-8 and IL-17A levels in the plateau group were higher(P<0.05),and the IL-6 and IL-8 levels in the returning to plain group were still higher than those in the plain group(P<0.05).Conclusion The levels of many blood biochemical and cytokines indexes among the young people immigrating to the plateau for 6 months to 1 year have changed,and the partial indexes in the young people separating from the plateau to return to the plain about 2 months do not recover to the levels in the young people living in the plain,indicating that the plateau hypoxia could affect the body metabolism function and induce the inflammatory reaction for a long time.