环介导等温扩增技术在SARS-CoV-2核酸检测中的应用研究

Application of loop-mediated isothermal amplification technique in nucleic acid detection of SARS-CoV-2

严重急性呼吸系统综合征冠状病毒2(SARS-CoV-2)的高度传染性导致全球SARS-CoV-2感染病例迅速增加。寻找一种较传统实时反转录聚合酶链反应(RT-PCR)技术更加准确、快速的检测方法对疾病确诊及疫情防控尤为重要。环介导等温扩增技术(LAMP)与反转录酶相结合(RT-LAMP)已成功应用于SARS与中东呼吸综合征的检测,在SARS-CoV-2检测中亦表现出潜在优势。该文对SARS-CoV-2的结构特点、人类感染机制、LAMP的检测原理及其在SARS-CoV-2检测中的应用研究进展进行详细综述,以期为临床提供一种较RT-PCR更加快速、特异且具有明显成本-效益的SARS-CoV-2核酸检测方法。

The high infectivity of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has led to a rapid increase in the cases of SARS-CoV-2 infection worldwide.It is particularly important to find a more accurate and rapid detection method than the traditional real-time reverse transcriptase polymerase chain reaction(RT-PCR)technique for the disease diagnosis and epidemic prevention and control.The loop-mediated isothermal amplification(LAMP)technique in combination with reverse transcriptase has been successfully applied for the detection of SARS and MERS,and also shows the potential advantage in the detection of SARS-CoV-2.This paper in detail reviews the structural features of SARS-CoV-2,human infection mechanism,detection principle of LAMP detection and the application study advances in the SARS-CoV-2 detection in order to provide a more rapid,more specific and obvious cost-benefit SARS-CoV-2 nucleic acid detection method for clinic.