基于JAK-STAT信号通路探讨槲皮素对宫颈癌细胞增殖、凋亡和转移的影响

Discussion on Effect of Quercetin on Proliferation,Apoptosis and Metastasis of Cervical Cancer Cells Based on JAK-STAT Signaling Pathway

目的:观察槲皮素对宫颈癌细胞增殖、凋亡和转移的影响,探讨其作用机制。方法:将宫颈癌C-33A细胞分为对照组及20、40、60μmol/L槲皮素组。采用MTT法检测细胞的增殖活力;流式细胞仪检测细胞的周期变化和凋亡率;Transwell法检测细胞的转移率;蛋白免疫印迹法检测蛋白酪氨酸激酶1(JAK1)、蛋白酪氨酸激酶2(JAK2)、信号传导及转录活化因子3(STAT3)。结果:与对照组比较,20、40、60μmol/L槲皮素组细胞增殖活力、转移率及S期、G2/M期细胞百分率明显降低(P<0.05),凋亡率、G0/G1期细胞百分率明显增加(P<0.05);且随着槲皮素浓度的升高,细胞的增殖活力、转移率及S期、G2/M期细胞百分率逐渐降低(P<0.05),凋亡率、G0/G1期细胞百分率明显增加,差异有统计学意义(P<0.05)。与对照组比较,20、40、60μmol/L槲皮素组JAK1、JAK2、STAT3蛋白水平明显降低,且随着槲皮素浓度的升高,JAK1、JAK2、STAT3蛋白水平逐渐降低,差异有统计学意义(P<0.05)。结论:槲皮素可通过JAK-STAT信号通路抑制宫颈癌细胞的增殖、转移,诱导其凋亡,阻滞细胞周期。

Objective:To observe the effect of quercetin on proliferation,apoptosis and metastasis of cervical cancer cells and discuss its mechanism.Methods:Cervical cancer C-33A cells were divided into the control group and the 20,40,60μmol/L quercetin groups.The MTT assay was used to detect the cell proliferation activity;the flow cytometry was used to detect the cycle change and apoptosis rate of cells;the Transwell assay was used to detect the cell transfer rate,and the Western blot was used to detect protein tyrosine kinase 1(JAK1),protein tyrosine kinase 2(JAK2),and signal transducer and activator of transcription 3(STAT3).Results:Compared with those in the control group,cell proliferation activity,metastasis rates and the percentage of cells in S phase and G2/M phase were significantly decreased in the20,40,60μmol/L quercetin groups(P<0.05),and the apoptosis rates and the percentage of cells in G0/G1 phase were significantly increased(P<0.05);with the increase of quercetin concentration,the proliferation activity,metastasis rates and the percentage of cells in S phase and G2/M phase were gradually decreased(P<0.05),and the apoptosis rates and the percentage of cells in G0/G1 phase were increased significantly(P<0.05),the differences being statistically significant.The protein levels of JAK1,JAK2 and STAT3 in the 20,40,60μmol/L quercetin group were significantly decreased when compared with those in the control group,and with the increase of quercetin concentration,the protein levels of JAK1,JAK2 and STAT3 were gradually decreased(P<0.05).Conclusion:Quercetin inhibits the proliferation and metastasis of cervical cancer,induces apoptosis and block cell cycle through JAK-STAT signaling pathway.