桃红四物汤对急性软组织损伤大鼠的保护作用及机制研究

Protective Effect and Mechanism of Taohong Siwu Decoction on Rats with Acute Soft Tissue Injury

目的研究桃红四物汤对急性软组织损伤大鼠的保护作用,并探讨其作用机制。方法采用重物打击法建立急性软组织损伤大鼠模型,将30只雄性SD大鼠分为5组:正常组、模型组、桃红四物汤低、高剂量组和阳性药物组,每组6只。桃红四物汤低、高剂量组分别给予4、12 g/kg桃红四物汤灌胃,阳性药物组给予45 mg/kg布洛芬灌胃,正常组和模型组给予等量生理盐水灌胃,每天1次,持续1周。治疗结束后处死大鼠。HE染色观察各组大鼠损伤组织病理变化。免疫组化观察各组大鼠损伤组织中转化生长因子-β1(TGF-β1)表达水平。生化检测各组大鼠损伤组织中超氧化物歧化酶(SOD)和丙二醛(MDA)水平。Western Blot检测各组大鼠损伤组织中IL-6、肿瘤坏死因子-α(TNF-α)及血管内皮生长因子(VEGF)蛋白表达水平。结果与正常组比较,模型组大鼠损伤部位淤血、肿胀明显,活动情况较差,损伤组织中可见出血,TGF-β1阳性表达减少,MDA浓度及IL-6、TNF-α蛋白表达水平显著升高(P<0.05),SOD活性及VEGF蛋白表达水平显著降低(P<0.05)。与模型组比较,桃红四物汤低、高剂量组大鼠损伤部位淤血、肿胀及活动情况好转,损伤组织中出血减少,TGF-β1阳性表达增多,MDA浓度及IL-6、TNF-α蛋白表达水平显著降低(P<0.05),SOD活性及VEGF蛋白表达水平显著升高(P<0.05)。结论桃红四物汤有助于急性软组织损伤大鼠的损伤修复,其作用机制与抑制炎症反应和氧化应激,促进TGF-β1、VEGF蛋白表达有关。

Objective To observe the protective effect of Taohong Siwu Decoction(THSWD)on acute soft tissue injury rats and to explore its mechanism.Methods The rat model of acute soft tissue injury was established by heavy blow method.Totally 30 male SD rats were divided into 5 groups:normal,model,low dose THSWD,high dose THSWD,positive drug group,6 rats in each group.Rats in low and high dose THSWD groups were respectively administered with 4 and 12 g/kg THSWD by gastrogavage.Rats in positive drug group were administered with 45 mg/kg ibuprofen by gastrogavage.The same amount of normal saline was administered to rats in normal and model groups by gastrogave.All administration was performed once a day for successive 1 week.The rats were sacrificed after treatment.HE staining was used to observe the pathological changes of injured tissues.Immunohistochemistry was used to observe the expression of transforming growth factor-β1(TGF-β1)in injured tissues.The levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in injured tissues were detected by biochemical method.Western Blot was used to detect the protein expression levels of interleukin 6(IL-6),tumor necrosis factor-α(TNF-α),and vascular endothelial growth factor(VEGF).Results Compared with the normal group,congestion and swelling,poor activity,bleeding in injured tissue could be obviously seen in model group.The positive expression of TGF-β1 decreased,the concentration of MDA and the protein expression levels of IL-6 and TNF-αsignificantly increased(P<0.05),SOD activity and VEGF protein expression level significantly decreased(P<0.05).Compared with the model group,congestion and swelling,and activity of injured parts in low and high dose THSWD groups were improved,bleeding in injured tissues was reduced,the positive expression of TGF-β1 increased,the concentration of MDA and the protein expression levels of IL-6 and TNF-αsignificantly decreased(P<0.05),SOD activity and VEGF protein expression level significantly increased(P<0.05).Conclusion THSWD was helpful to the repair of acute soft tissue injured rats.Its mechanism might be related to inhibiting inflammatory reactions and oxidative stress,and promoting the expressions of TGF-β1 and VEGF.