不同种源银柴胡种子质量及幼苗抗逆性综合评价

Comprehensive Evaluation of Seed Quality and Seedling Stress Resistance of Stellaria dichotoma L.var.lanceolata Bge.from Different Provenances

为研究不同种源银柴胡种子质量及抗逆性,为道地产区银柴胡规模化种植提供优质种源材料。测定采自不同种源地的13份银柴胡种子材料的千粒重、种子活力、不同温度下的发芽率等种子质量指标,以及大田试验植株幼苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、丙二醛(MDA)、膜透性、脯氨酸(Pro)、可溶性蛋白等抗逆生理指标,对种子质量指标和幼苗抗逆生理指标进行主成分综合评价。结果表明,13份银柴胡种源材料中85%的种子生活力在95%以上,62%的种子千粒重在1.45~1.65 g,种子最适发芽温度为20~25℃。13份银柴胡种源材料综合排名依次为3号、1号、5号、2号、9号、11号、7号、10号、4号、8号、13号、6号、12号。综合排名靠前的有种源3号、1号、5号、2号,说明这4个种源银柴胡种子质量较好、抗逆性强。

To study the seed quality and stress resistance of Stellaria dichotoma L.var.lanceolata Bge.from different provenances and to provide high-quality provenance materials for large-scale cultivation of Stellaria dichotoma in genuine producing area,this study measured seed quality indicators such as thousand-seed weight,seed viability,and germination rate at different temperatures on 13 samples of Stellaria dichotoma seed material collected from different provenances,as well as field tests to determine plant physiological indicators of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),malondialdehyde(MDA),membrane permeability,proline(Pro)and soluble proteins.Principal component comprehensive evaluation was performed on the seed quality indexes and physiological stress indexes of seedling of Stellaria dichotoma.Results showed that among the 13 materials of Stellaria dichotoma,85%of the seeds had a viability of more than 95%,62%of the seeds had thousand-seed weights of 1.45 to 1.65 g,and the optimal germination temperature of the seeds was 20 to 25℃.13 Stellaria dichotoma materials were ranked comprehensively as No.3>No.1>No.5>No.2>No.9>No.11>No.7>No.10>No.4>No.8>No.13>No.6>No.12.Top rankings were provenances No.3,No.1,No.5,and No.2,indicating that these four provenances have good seed quality and strong resistance.