半夏叶柄组织培养一步成苗研究

Study on One-step Plantlet Formation of Pinellia ternate by Petiole Tissue Culture

为给半夏组织培养和工厂化育苗体系的建立提供参考,以半夏叶柄为外植体,研究了不同消毒措施以及不同浓度激素对诱导外植体一次性成苗的影响。结果显示,以75%乙醇消毒30 s,再用0.1%HgCl溶液消毒6 min的灭菌效果最好;最佳诱导培养基为MS+蔗糖30 g/L+琼脂8 g/L+6-BA 1.0 mg/L+NAA 0.2 mg/L。

In order to provide reference for the establishment of tissue culture system and factory seedling system of Pinellia ternate,the petiole of Pinellia ternate was used as explants to study the effects of different disinfection measures and different concentrations of hormones on inducing one-step plantlet formation.The results showed that the best sterilization measure was to sterilize with 75%ethanol for 30 s,and then sterilize with 0.1%HgCl solution for 6 min;The best induction medium was MS+sucrose 30 g/L+agar 8 g/L+6-BA 1.0 mg/L+NAA 0.2 mg/L.