摘要
目的评估中性粒细胞外捕网(neutrophil extracellular traps,NETs)在胃癌病人术后高凝状态形成中的作用。方法抽取2018年4月至2021年6月双鸭山双矿医院普外科手术的胃癌病人21例,另选取健康志愿者21名为对照,检测外周血浆中髓过氧化物酶-DNA复合体(MPO-DNA)、凝血酶-抗凝血酶复合物(thrombin-antithrombin complex,TAT)含量;采用胃癌术后病人血浆与体外培养的正常人中性粒细胞共孵育,检测NETs含量;在DNA酶-1干预下反向验证NETs对术后TAT的影响。结果与对照组及胃癌病人术前比较,胃癌病人术后外周血中D-二聚体及TAT明显升高,NETs生成明显增加(P<0.01);术后病人血浆可刺激正常人中性粒细胞释放NETs(P<0.01),DNA酶-1可通过分解NETs,抑制胃癌术后TAT水平(P<0.01)。结论NETs生成增加可能为胃癌病人术后高凝状态形成诱因之一。
Objective To evaluate the procoagulant activity(PCA)of neutrophil extracellular traps(NETs)in gastric cancer(GC)patients after surgery.Methods NETs formation and their PCAs were assessed with GC before and after surgery using enzyme-linked immunosorbent assay(ELISA)and immunofluorescent microscopy.Results GC patients after surgery displayed significantly elevated levels of D-dimers and thrombin-antithrombin(TAT)complex as well as NETs formation compared with those from healthy controls and patients pre-operation.Moreover,plasma from GC patients after surgery induced control neutrophils to release NETs.Notably procoagulant effects of GC patients after surgery were dramatically attenuated by application of DNAse-Ⅰ.Conclusion NETs may contribute to the coagulopathy of GC patients after surgery.
作者
杨春发
刘胜超
王金帅
赵德萍
雷霞
崔云甫
Yang Chunfa;Liu Shengchao;Wang Jinshuai;Zhao Deping;Lei Xia;Cui Yunfu(Department of General Surgery,Shuangkuang Hospital,Heilongjiang Shuangyashan 155100,China;Heilongjiang University of Traditional Chinese Medicine,Heilongjiang Harbin 155000,China;Department of General Surgery,Second Affiliated Hospital,Harbin Medical University,Heilongjiang Harbin 155000,China)
出处
《腹部外科》
2022年第6期427-429,440,共4页
Journal of Abdominal Surgery
基金
黑龙江卫生计生委科研课题(2018356)。
关键词
胃癌
术后高凝状态
中性粒细胞外捕网
Gastric cancer
Hypercoagulable state
Neutrophil extracellular traps
