接触辉光放电等离子体对Actinomucor elegans的杀菌作用与机理研究

Study on sterilization effects and mechanisms of contact glow discharge plasma on Actinomucor elegans

雅致放射毛霉(Actinomucor elegans)感染的毛霉病在人群中比较严重,如何杀灭Actinomucor elegans显得尤为重要。为了研究接触辉光放电等离子体(CGDP)对Actinomucor elegans的杀菌作用与机理,本文通过考察电压、时间、电解质对杀菌效率的影响,确定了杀菌条件。通过分析CGDP对菌落形态、孢子内容物泄漏和过氧化损伤的影响,探讨了CGDP对Actinomucor elegans的杀菌机理。结果表明:CGDP对Actinomucor elegans的杀菌效果显著,在560 V、15 min、Na_(2)SO_(4)溶液的条件下杀菌率(η)最高达90.71%;CGDP处理后菌落直径、孢子萌发率、菌丝生物量积累等明显减小;孢子内容物泄漏量、过氧化损伤程度等显著增大;孢子表面皱缩甚至破裂。初步确定,CGDP通过破坏细胞膜完整性使Actinomucor elegans失活。本文结果为CGDP在杀灭真菌领域的应用及机理研究提供了一定的参考依据。

Mucormycosis infected by Actinomucor elegans is relatively serious in the population, so it is urgent need to inactivate Actinomucor elegans. In order to investigate the sterilization effect and mechanisms of contact glow discharge plasma(CGDP) on Actinomucor elegans, the effects of voltage, time and electrolyte on sterilization efficiency were investigated, and the sterilization conditions were confirmed. The sterilization mechanism of CGDP on Actinomucor elegans was explored by analyzing the changes of colony morphology, spore content leakage and peroxidation damage that induced by CGDP treatment. The results showed that CGDP had remarkable sterilization effects on Actinomucor elegans, under the conditions of 560 V, 15 min and Na_(2)SO_(4)solution, the sterilization rate(η)was up to 90.71%, the colony diameter, spore germination rate and mycelial biomass accumulation all decreased significantly after CGDP treatment, while leakage of intracellular components and degree of peroxidation damage increased, and spore surface became shrinking and rupturing. It was concluded that the Actinomucor elegans was inactivated due to cell membrane integrity destroying induced by CGDP. The results of this paper could provide a reference for the application and mechanism research of CGDP in the field of fungicidal.