脑源性神经营养因子在下咽鳞状细胞癌中的表达及临床意义

Expression and significance of BDNF in hypopharyngeal squamous cell carcinoma

目的探讨脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)在下咽鳞状细胞癌(Hypopharyngeal squamous cell carcinoma,HSCC)组织中的表达及其与HSCC患者临床病理学特征的关系,分析BDNF与下咽癌侵袭转移能力的关系。方法收集2013年4月-2016年12月HSCC患者33例的病理和临床资料,免疫组织化学染色(IHC)方法检测33例HSCC患者癌组织、22例癌旁组织及12例非癌患者正常咽部黏膜上皮中BDNF的表达水平。Transwell侵袭实验检测外源性BDNF对下咽癌FaDu细胞系侵袭能力的影响。结果BDNF蛋白在HSCC癌组织中的表达显著高于癌旁组织及正常咽部黏膜组织,差异具有统计学意义(P<0.0125);BDNF蛋白在HSCC癌旁组织中的表达明显高于正常咽部黏膜组织,差异具有统计学意义(P<0.0125);HSCC癌组织标本中BDNF蛋白的表达与肿瘤直径、分化程度、是否淋巴结转移及临床分期相关(P<0.05),与性别、年龄及浸润深度无关(P>0.05);Transwell侵袭实验添加外源性BDNF的实验组对比未添加外源性BDNF的对照组,外源性BDNF明显增强FaDu细胞的侵袭能力,两组差异具有统计学意义(P<0.05)。结论BDNF蛋白在HSCC癌组织中表达水平与肿瘤直径、分化程度、淋巴结转移及临床分期相关;BDNF蛋白可促进FaDu细胞的侵袭转移能力。

Objective To investigate the expression of brain-derived neurotrophic factor(BDNF)in hypopharyngeal squamous cell carcinoma(HSCC)and its relationship with clinicopathological features of HSCC,and to analyze the relationship between BDNF and invasion and metastasis of hypopharyngeal carcinoma.Methods The pathological and clinical data of 33 patients with HSCC who underwent surgery in the Department of Otorhinolaryngology of our hospital from April 2013 to December 2016 were collected.Immunohistochemical staining(IHC)was used to detect the expression of BDNF in 33 cases of HSCC patients,22 cases of paracancerous tissues and 12 cases of normal pharyngeal mucosa.Transwell invasion assay was used to detect the effect of BDNF on the invasive ability of hypopharyngeal carcinoma FaDu cell line.Results Immunohistochemical staining showed that the expression of BDNF protein in cancer tissues was significantly higher than that in paracancerous tissues and normal pharyngeal mucosa,and the difference had statistical significance(P<0.0125);the expression of BDNF protein in paracancerous tissues was significantly higher than that in normal pharyngeal mucosa,and the difference had statistical significance(P<0.0125).The expression of BDNF protein in cancer tissues was correlated with tumor diameter,degree of differentiation,lymph node metastasis and clinical stage(P<0.05),but not with sex,age and depth of invasion(P>0.05).Transwell invasion assay found that the FaDu cell invasion ability was significantly enhanced in the experimental group supplemented with exogenous BDNF compared with the control group without exogenous BDNF,and the difference had statistical significance(P<0.05).Conclusion The expression level of BDNF protein in cancer tissues is significantly higher than that in paracancerous tissues and normal pharyngeal mucosa,and the expression level is related to tumor diameter,degree of differentiation,lymph node metastasis and clinical stage.BDNF protein can promote the invasion and metastasis of FaDu cells.