二次冻融胚胎移植后的临床结局

Clinical outcomes after twice frozen-thawed embryo transfer

目的探讨二次冻融对胚胎移植临床结局的影响。方法收集2015年—2020年在深圳市罗湖区人民医院生殖医学科行二次冻融胚胎移植的48个周期为观察组,随机选取同期实施一次冻融胚胎移植的98个周期为对照组。比较两组年龄、内膜厚度、移植胚胎数、移植优质胚胎数、胚胎复苏率、种植率、临床妊娠率、流产率、异位妊娠率及活产率。结果两组患者年龄、内膜厚度、移植胚胎数与移植优质胚胎数比较差异均无统计学意义(均P>0.05)。两组胚胎复苏率、异位妊娠率、流产率与活产率比较差异无统计学意义(均P>0.05)。观察组的胚胎种植率与临床妊娠率显著低于对照组(32.47%vs 55.70%,39.58%vs 66.33%,均P<0.05)。结论胚胎经过二次冻融再移植,临床妊娠率明显低于对照组,反复冻融可能影响了胚胎后续发育潜能。

Objective To investigate the effect of repeated freezing and thawing on the clinical outcome of embryo transfer.Methods A total of 48 cycles of twice frozen-thawed embryo(blastocyst)transfer in the reproductive medicine department,Shenzhen Luohu People′s Hospital from 2015 to 2020 were collected as the observation group,and 98 cycles of one frozen-thawed blastocyst transfer in the same period were randomly selected as the control group.The patient′s age,endometrial thickness,average number of transferred embryos,average number of high-quality embryos transferred,embryo recovery rate,implantation rate,clinical pregnancy rate,abortion rate,ectopic pregnancy rate,and live birth rate were compared.Results There was no significant difference in age,endometrial thickness,number of embryos transferred and high-quality embryos transferred between the two groups(all P>0.05).There was no significant difference in embryo recovery rate,ectopic pregnancy rate,abortion rate and live birth rate between the two groups(all P>0.05).The embryo implantation rate and clinical pregnancy rate in the observation group were significantly lower than those in the control group(32.47%vs 55.70%,39.58%vs 66.33%,all P<0.05).Conclusions The clinical pregnancy rate was significantly lower than that of the control group after repeated vitrification of frozen-thawed embryo transfer,which may affect the subsequent developmental potential of embryos.