5%香芹酚水剂对典型环境生物的急性毒性及生长影响评价

Evaluation of acute toxicity and growth impact of carvacrol 5% aqueous solution to typical environmental organisms

[目的]为了明确5%香芹酚水剂对环境生物的安全性,开展了该药剂对有益非靶标生物鹌鹑、蜜蜂、家蚕、斑马鱼、大型溞及藻类的急性毒理试验及生长影响评价。[方法]利用饲喂法、浸叶法、点滴法、静态实验法、寇式法等试验方法评价5%香芹酚水剂对环境生物的毒性及影响。[结果]5%香芹酚水剂对鹌鹑、家蚕、蜜蜂接触毒性的LD_(50)值分别为>400 mg a.i./kg、1064 mg a.i./L、11.0μg a.i./bee;对斑马鱼急性毒性和蜜蜂经口毒性的LC值分别为1.93、601 mg a.i./L;对大型溞急性毒性的EC_(50)值为0.953 mg a.i./L;对斜生栅藻生长抑制的EyC值为4.57 mg a.i./L。[结论]该药剂对鹌鹑、家蚕、蜜蜂和斜生栅藻表现为低毒,对斑马鱼表现为中毒,对大型溞表现为高风险性,对环境安全性较好。

[Aims] This study aims to confirm safety of carvacrol 5% aqueous solution to environmental organisms, the acute toxicological test and growth impact evaluation of beneficial non-target organisms such as quail, bees, silkworms,zebrafish, macrocoasters and algae were carried out. [Methods] For the first time, experimental methods such as feeding method, leaf soaking method, drip method, static experimental method, and Kou method were used to evaluate the toxicity and effect of 5% carvacrol aqueous solution to environmental organisms. [Results] The results showed that the acute contact toxicity LD_(50)values of carvacrol 5% aqueous solution to Coturnix coturnix Japonica, Bombyx mori and Apis mellifera L. were >400 mg a.i./kg, 1064 mg a.i./L and 11.0 μg a.i./bee, respectively. The acute oral toxicity LCvalues to Brachydonio rerio and A. mellifera L. were 1.93 and 601 mg a.i./L, respectively. The acute toxicity EC_(50) to Daphnia magna Straus was 0.953 mg a.i./L. The inhibitory activity EyCto Scenedesmus obliquus was 4.57 mg a.i./L.[Conclusions] The pesticide is low toxicity to C. coturnix Japonica, B. mori, A. mellifera L. and S. obliquus, moderate toxicity to B. rerio, high toxicity to D. magna Straus, which is relatively safe to the environment.