紫菜复合酶解液改善饮食诱导肥胖和调节肠道菌群作用

Effect of Complex Enzymatic Hydrolysate of Porphyra on Improving High Fat Diet-induced Obesity and Regulating Intestinal Flora

使用中性蛋白酶和自制紫菜多糖酶降解坛紫菜,制备紫菜复合酶解液。结合16S rRNA测序、气相色谱检测、荧光定量PCR分析,探究两种酶解液对饮食诱导肥胖和肠道菌群的影响。结果表明,紫菜复合酶解液能有效调节高脂饮食小鼠血清脂质水平,降低白色脂肪含量和脂肪细胞大小。这可能是由于复合酶解液可以抑制肝脏中脂肪合成相关基因(DGAT1、DGAT2)的表达,促进脂肪组织中脂肪分解相关基因(AGTL、HSL)的表达。同时,紫菜复合酶解液促进有益菌Muribaculaceae和螺杆菌科的增殖,抑制有害菌链球菌科的生长,并上调“甘油酯代谢”等脂代谢相关通路。总之,紫菜复合酶解液能有效改善饮食诱导的肥胖,调节与肥胖相关的肠道菌群组成和功能。复合酶解液有潜力用于新型功能饮料的开发,有助于促进紫菜的高值化利用。

The compound enzymatic hydrolysate of Porphyra(CPEH)was prepared using a novel porphyranase(EC 3.2.1.178)and neutral protease.Then 16S r RNA gene sequencing,gas chromatography and real-time PCR analysis were used to analyze the effects of CPEH on diet-induced obesity(DIO)and gut microbiome in HFD-fed mice.The results suggested that CPEH improved blood lipid levels,alleviated body fat deposition,and reduced adipocyte size.Possibly because CPEH could repress the expression of genes(DGAT1 and DGAT2)related to triglyceride synthesis in liver,and promote gene expression of enzymes(ATGL and HSL)related to lipolysis in adipose tissue.Furthermore,CPEH promoted the proliferation of beneficial bacteria(Muribaculaceae and Helicobacteraceae)related to obesity and repressed the growth of harmful bacteria(Streptococcaceae).CPEH also regulated the abundance of KEGG pathways associated with lipid metabolism,such as’Glycerolipid_metabolism’.In summary,CPEH effectively prevented high fat diet-induced obesity and regulated gut microbial composition and function.CPEH has potential to be applied to the development of functional beverage.This study could contribute to the high value application of low valued Porphyra.