青稞对(60)^Co-γ射线照射小鼠造血功能的保护作用

Protective Effects of Highland Barley on Hematopoietic Function in Mice Irradiated with(60)^Co-γRay

目的:探讨青稞对辐射小鼠造血功能的保护作用。方法:以含20%青稞饲料饲养C57BL/6J小鼠12周后,采用4 Gy剂量(60)^Co-γ射线照射全身。照射后继续干预2周,监测其体重、摄食量、外周血象、骨髓与脾脏组织形态、骨髓有核细胞数、脏器指数及血生化水平,以评价其造血功能恢复情况。结果:与辐射对照组相比,青稞干预后小鼠在辐照第4天体重明显恢复,第1周摄食量显著上升,总胆固醇(TC)水平明显恢复,小鼠的骨髓有核细胞数、红细胞、血红蛋白、白细胞及血淋巴细胞水平明显恢复,且小鼠骨髓结构损伤较小。同时,青稞干预可有效恢复辐照导致的脏器指数的异常并保护脾脏结构。本研究结果表明,青稞的预防性干预通过减轻小鼠辐照后脾脏组织形态的损伤、白细胞、淋巴细胞、脏器指数及血生化水平的异常来防止对免疫功能的损伤。通过恢复小鼠辐照后骨髓结构的损伤,促进骨髓有核细胞数及外周血红细胞的恢复来保护造血功能。结论:青稞预防性干预是通过保护机体免疫功能,有效恢复辐射引起的造血功能损伤。

Objective:To explore the protective effect of highland barley(Hordeum vulgare L.var.nudum Hook.F.)on the hematopoietic function of irradiated mice.Methods:After feeding with 20%highland barley diet for 12 weeks,C57BL/6J mice were irradiated with(60)^Co-γrays at a dose of 4 Gy once,then continue to raise two more weeks.The body weight,food intake,peripheral blood image,bone marrow and spleen tissue morphology,bone marrow nucleated cell count,organ index and blood biochemical level were monitored to evaluate the recovery of hematopoietic function.Results:Compare with irradiation group,the body weight of the mice in highland barley intervention group was significantly recovered on the 4thday after irradiation,the food intake in the 1stweek was significantly increased.Also,total cholesterol(TC),bone marrow nucleated cells,red blood cells,hemoglobin,white blood cells,and blood lymphocyte cell levels were significantly recovered,and the bone marrow structure had no significant damage.At the same time,highland barley intervention could effectively prevent the abnormal organ index and protect the spleen structure after irradiation.The studies have shown that the preventive intervention of highland barley can prevent the damage to immune function by reducing the damage of spleen tissue morphology,the abnormality of white blood cells,lymphocytes,organ indexes and blood biochemical levels after irradiation.And protect hematopoietic function by recovering the damage of bone marrow structure in mice after irradiation,and promoting the recovery of bone marrow nucleated cells and peripheral blood red blood cells.Conclusion:The preventive intervention of highland barley is to effectively recover the hematopoietic damage caused by radiation through protecting the immune function of the body.