鲍鱼肌肉蛋白对Lewis肺癌化疗小鼠短期营养支持作用研究

Short-term Nutritional Support of Abalone Muscle Protein on Lewis Lung Cancer Chemotherapy Mice

目的:探讨鲍鱼肌肉蛋白对化疗肿瘤小鼠营养状况的影响。方法:构建Lewis肺癌化疗小鼠模型,给予鲍鱼肌肉蛋白作为营养支持,以体质量、肿瘤体积、脏器指数(脾脏指数和胸腺指数)以及血清中蛋白因子水平【白蛋白(ALB)、前白蛋白(PA)、血红蛋白(Hb)】为指标,对比饲喂蛋白前、后相关指标的变化。结果:小鼠皮下注射Lewis肺癌细胞7 d后有明显肿块;生存状况显示化疗质量浓度为0.02 mg/mL和0.04 mg/mL的小鼠无死亡率,考虑生存周期选用0.02 mg/mL为最适化疗药物浓度。在营养支持过程中中剂量组的肿瘤体积明显减小,抑瘤率达20.7%。与空白组(NT)对比,高剂量组可以提高化疗小鼠的脾脏指数和胸腺指数(P>0.05)。营养指标显示,与空白组(NT)对比,对化疗小鼠进行营养支持后,各组的ALB、PA水平均有明显升高,且低剂量组的PA水平显著升高(P<0.05),Hb水平明显降低(P<0.05)。结论:鲍鱼肌肉蛋白对化疗肿瘤小鼠营养状况具有一定的改善作用。

Objective:To investigate the effect of abalone muscle protein on the nutritional status of mice with chemotherapy tumors.Methods:The Lewis lung cancer chemotherapy mouse model was constructed,and provided nutritional support by giving abalone muscle protein to body weight,tumor volume,organ index(spleen index and thymus index),and serum.The levels of protein factors ALB,PA and Hb are used as indicators,and the numerical changes of related indicators before and after nutritional support are compared.Results:The mice had obvious lumps after subcutaneous injection 7 days;the survival status showed that the cisplatin chemotherapy mass concentrations of 0.02 mg/mL and0.04 mg/mL had no mortality.Considering the survival period,0.02 mg/mL was selected as the optimal concentration of chemotherapeutics.During the nutritional support process,the tumors of the sample in the dose group were significantly reduced,and the tumor inhibition rate reached 20.7%.Compared with the blank group,the sample high-dose group can increase the spleen index and thymus index of chemotherapy mice(P>0.05).Nutritional index results showed that after nutritional support for chemotherapy mice,comparison with blank group(NT),the ALB and PA of each group were significantly increased,and the PA of the sample low-dose group was significantly increased(P<0.05),the Hb was significantly reduced(P<0.05).Conclusion:AMPP has a certain effect on improving the nutritional status of mice with tumor chemotherapy.