牛Basigin基因变异体的克隆与鉴定

Cloning and identification of bovine Basigin gene variants

为了研究牛Basigin(BSG)基因是否存在变异体,采用3′RACE和测序技术对牛的BSG基因进行克隆和鉴定,成功克隆出了牛BSG基因4条转录本的3′端片段,分别命名为BSG-v1、BSG-v2、BSG-v3和BSG-v4,其中BSG-v2、BSG-v3和BSG-v4是新发现的牛BSG基因变异体。研究表明,BSG-v2在第7外显子处发生了3′端可变剪接,导致BSG-v2的外显子7比BSG-v1的短了515 bp,但没有改变BSG基因的开放阅读框;BSG-v3和BSG-v4都发生了选择性多聚腺苷酸化,由于没有外显子7,导致产生了更短的3′UTR,说明牛的BSG基因存在不同形式的变异体,这为进一步研究牛BSG基因在不同生物学过程中的功能提供了分子生物学基础。

To investigate whether there are variants of the bovine Basigin(BSG)gene,the 3′ RACE and sequencing techniques were used to clone and characterize the bovine BSG gene,and the 3′ end fragments of four transcripts of the bovine BSG gene,named BSGv1,BSG-v2,BSG-v3 and BSG-v4,were successfully cloned,of which BSG-v2,BSG-v3 and BSG-v4 were newly discovered variants of the bovine BSG gene. It was shown that BSG-v2 underwent variable splicing at exon 7,resulting in exon 7 of BSG-v2 being 515bp shorter than that of BSG-v1,but did not change the open reading frame of BSG gene. Both BSG-v3 and BSG-v4 underwent alternative polyadenylation,which resulted in a shorter 3′ UTR due to the absence of exon 7,indicating the existence of different forms of variants of the bovine BSG gene. It provides a molecular biological basis for further studies on the function of the bovine BSG gene in different biological processes.