基于PEG胁迫的不同棉花资源幼苗根系特征与抗旱性鉴定

Root characteristics and drought resistance identification of different cotton resources seedlings based on PEG stress

为研究棉花(Gossypium spp.)苗期根部性状在干旱胁迫下的表现,以邯郸市农业科学院自育17个耐旱性不同的棉花品系为材料,通过PEG6000干旱胁迫,对不同棉花资源的根系特征进行比较。采用根系图像扫描和WinRHIZO软件进行根系分析,比较不同品种的根长度相对增长率、根表面积相对增长率、根系平均直径相对增长率等5个指标,采用方差分析、多重比较、系统聚类等统计分析方法,判别供试品种的抗旱性。结果表明,不同棉花品种在质量分数为15.0%的PEG6000胁迫下,抗旱性存在一定差异。依聚类分析和抗旱性表现可将棉花品种分成3类,第一类为HL64;第二类包括HL28、HL59、HL66、HL60;第三类为HL31、HL47、HL68、HL27、HL45、HL33、HL44、HL29、HL69、HL34、HL43、HL42。

In order to study the performance of root traits of cotton(Gossypium spp.)seedlings under drought stress,17 cotton lines with different drought tolerance were bred by Handan Academy of Agricultural Sciences.The root characteristics of different cotton re⁃sources were compared by PEG6000 drought stress.The root image scanning and WinRHIZO software were used to analyze the root system.The relative growth rate of root length,relative growth rate of root surface area and relative growth rate of root average diameter of different varieties were compared.The drought resistance of the tested varieties was determined by variance analysis,multiple com⁃parison and systematic clustering.The results showed that there were some differences in drought resistance of different cotton varieties under 15.0%PEG6000 stress.According to cluster analysis and drought resistance performance,cotton varieties could be divided into 3 categories:the first category was HL64;the second category included HL28,HL59,HL66,HL60;the third category was HL31,HL47,HL68,HL27,HL45,HL33,HL44,HL29,HL69,HL34,HL43,and HL42.