白背叶中黄酮类成分分离鉴定及其含量测定

Isolation identification and content determination of flavonoids in Ricinus apelta

分离鉴定不同产地白背叶(Ricinus apelta Lour.)中黄酮类化合物成分,并建立其含量测定方法。采用聚酰胺柱,基于高效液相色谱法对白背叶中黄酮类化学成分进行分离纯化,应用红外、紫外、质谱和核磁共振波谱法对其化学成分结构进行鉴定,同时测定4种成分含量。结果表明,从白背叶乙酸乙酯提取物中分离得到4种黄酮类化合物,经鉴定为葫芦芭苷Ⅱ、异夏佛托苷、夏佛托苷、大波斯菊苷;含量测定4种成分均达到基线分离,分别在0.0103~0.8240 mg/mL、0.0142~1.1392 mg/mL、0.0133~1.0640 mg/mL、0.0155~1.3240 mg/mL水平上线性关系良好;加样回收率分别为98.41%、100.03%、99.25%、98.78%,RSD分别为1.84%、0.90%、1.80%、1.25%;异夏佛托苷和夏佛托苷为首次从白背叶中分离得到,广西壮族自治区7个不同产地白背叶中,崇左市和贺州市4种黄酮类化合物含量总和较高。建立的含量测定方法操作简单快捷、稳定性好,可以作为白背叶质量评价的指标。

The flavonoids in Ricinus apelta Lour.from different habitats were isolated and identified,and the content determination method was established.Polyamide column was used to isolate and purify the flavonoids from Ricinus apelta by high performance liquid chromatography.The chemical constituents were identified by infrared,ultraviolet,mass spectrometry and nuclear magnetic resonance spectroscopy,and the contents of the four components were determined.The results showed that four flavonoids were isolated from the ethyl acetate extract of Ricinus apelta Lour.,which were identified as cucurbitacin II,isoschaftoside,schaftoside and cosmoside.The baseline separation of the four components was achieved in the content determination,and the linear relationship was good at the levels of 0.0103~0.8240 mg/mL,0.0142~1.1392 mg/mL,0.0133~1.0640 mg/mL and 0.0155~1.3240 mg/mL,respectively.The average recovery was 98.41%,100.03%,99.25% and 98.78%,respectively,and RSD was 1.84%,0.90%,1.80% and 1.25%,respectively.Isoschaftoside and schaftoside were isolated from Ricinus apelta Lour.for the first time.The contents of four flavonoids in Chongzuo and Hezhou were higher in Ricinus apelta Lour.from seven different habitats in Guangxi Zhuang Autonomous Region.The established content determination method is simple,rapid and stable,and can be used as an index for quality evaluation of Ricinus apelta Lour..