具核梭杆菌诱导自噬调控蛋白LC3高表达在食管鳞癌中的临床意义

The clinical significance of high expression of autophagy regulatory protein LC3 induced by Fn in ESCC

目的分析具核梭杆菌(Fn)对食管鳞癌(ESCC)细胞中自噬调控蛋白LC3的诱导效应,并探讨其临床意义。方法建立LC3敲降的KYSE150细胞系。采用Western blot、平板克隆、划痕实验及Transwell检测Fn及LC3对KYSE150细胞恶性生物学行为的影响。通过RNAscope及免疫组化检测ESCC组织中Fn感染及LC3表达情况。采用Kaplan-Meier法分析二者对患者生存期的影响。结果KYSE150细胞感染Fn时,LC3表达量增加,恶性生物学行为增强;LC3敲降时恶性生物学行为减弱(均P<0.05)。ESCC组织中Fn感染与LC3高表达具有一致性(P<0.05)。Fn感染及LC3表达阳性组患者生存期缩短(P<0.05)。结论Fn可通过诱导ESCC细胞中LC3高表达,富集自噬小体,为自身定植提供条件,促进ESCC恶性进展。

Objective To analyze the induction effect of Fusobacterium nucleatum(Fn)on autophagy regulatory protein LC3 in esophageal squamous cell carcinoma(ESCC)cells and explore its clinical significance.Methods The KYSE150 cell line with LC3 knockdown was established.Western blot,plate cloning,scratch test and Transwell were used to detect the effects of Fn and LC3 on the malignant biological behavior of KYSE150 cells.Fn infection and LC3 expression in ESCC tissues were detected by RNAscope and immunohistochemistry.KaplanMeier method was used to analyze the effects of Fn infection and LC3 expression on the survival phasein patients.Results As KYSE150 cells were infected with Fn,the expression of LC3 increased and the malignant biological behavior enhanced;following LC3 was knocked down,the malignant biological behavior reduced(P<0.05).Infection of Fn in ESCC tissues was consistent with high expression of LC3(P<0.05).The survival phase was shortened in the patients with both Fn infection and positive expression of LC3(P<0.05).Conclusions Fn can accelerate malignant progression of ESCC by inducing high LC3 expression in ESCC cells,enriching autophagosomes,and providing conditions for self-colonization.