番茄JAZ家族对病原菌诱导响应及成员间互作的分析

Analysis of the Induced Response and Interaction Among Tomato JAZ Family Members to Pathogens

茉莉酸(jasmonate acid,JA)在植物生长发育及防御过程中发挥着重要作用,如叶片衰老、花粉萌发、果实成熟、气孔关闭等。Jasmonate ZIM-domain蛋白(JAZ)是茉莉酸信号途径的抑制子,主要通过结合下游转录因子发挥调控JA响应的功能。表达模式分析发现,SlJAZs均能在一定程度上响应Pst DC3000的诱导。越来越多的研究发现,JAZ成员间通过互作来共同调控茉莉酸介导的植物生理过程。为揭示番茄JAZ家族成员相互作用的情况,以番茄JAZ家族13个基因为研究对象,利用酵母双杂交技术构建番茄JAZ互作网络,研究13个SlJAZ蛋白之间的互作情况。结果表明,SlJAZ1和SlJAZ2、SlJAZ5、SlJAZ6、SlJAZ7、SlJAZ10、Sl JAZ11;SlJAZ2和SlJAZ11;SlJAZ4和Sl JAZ6、SlJAZ10;SlJAZ5和SlJAZ6、SlJAZ8;SlJAZ6和SlJAZ10、Sl JAZ11、SlJAZ13;SlJAZ7和SlJAZ11;SlJAZ8和SlJAZ11之间存在相互作用,利用双分子荧光互补实验(bimolecular fluorescence complement,BiFC)进一步验证其互作关系。结果表明,SlJAZs可能通过蛋白互作的形式影响其他成员发挥作用。本研究初步明确了番茄JAZ家族成员的互作情况,对于研究JAZ成员协同调控茉莉酸信号通路具有重要的意义。

Jasmonic acid plays an important role in plant growth,development,and defense,including leaf senescence,pollen germination,fruit ripening,stomatal closure,and so on.It also activates the plant defense responding to both biological and abiotic stresses.JAZ is an inhibitor of jasmonic acid signaling pathway,which regulates JA response by binding to downstream transcription factors.Expression pattern analysis showed that Sl JAZs could respond to Pst DC3000.More and more studies have found that JAZ proteins interact with each other to regulate jasmonic acid-mediated plant physiological processes.In order to reveal the interaction of tomato JAZ family members,13 genes of tomato JAZ family were taken as the research object,and the tomato JAZ interaction network was constructed by yeast two hybrid technology to study the interaction between 13 SlJAZ proteins.These results showed that SlJAZ1 interacts with Sl JAZ2,SlJAZ5,SlJAZ6,SlJAZ7,SlJAZ10,SlJAZ11;Sl JAZ2 interacts with SlJAZ11;SlJAZ4 interacts with SlJAZ6,SlJAZ10;SlJAZ5 interacts with SlJAZ6,SlJAZ8;Sl JAZ6 interacts with Sl JAZ10,SlJAZ11,SlJAZ13;SlJAZ7 interacts with SlJAZ11;SlJAZ8 interacts with Sl JAZ11.The interaction was further verified by bimolecular fluorescence complementation experiment.And the results suggest that SlJAZs may influence other members to function through protein interactions.This study preliminarily clarified the interaction of JAZ family members in tomato,which is of great significance for studying the synergistic regulation of jasmonic acid signaling pathway by JAZ members.