血管内皮生长因子-C对鼻咽癌CNE-2细胞小鼠移植瘤的辐射敏感性的影响

Effect of VEGF-C on Radiation Sensitivity of Nasopharyngeal Carcinoma CNE-2 Cell Transplanted Mice

目的探讨干扰VEGF-C表达可以增加鼻咽癌CNE-2细胞小鼠移植瘤辐射敏感性作用以及相关信号途径。方法鼻咽癌CNE-2小鼠移植瘤随机分为:瘤体组;siRNA-NC组;siRNA-VEGF-C组;照射组;siRNA-NC+照射组;siRNA-VEGF-C+照射组。观察肿瘤生长情况;采用免疫组化检测VEGF-C、ATM、P65蛋白的表达变化。结果干扰VEGF-C表达鼻咽癌CNE-2细胞小鼠移植瘤,在照射后明显肿瘤生长明显变慢。免疫组化检测结果显示:干扰VEGF-C小鼠移植瘤后,VEGF-C、ATM明显减少(P<0.05),P65增加(P<0.05);联合照射后,VEGF-C、ATM明显减少(P<0.05)。结论在鼻咽癌CNE-2细胞小鼠移植瘤中,干扰VEGF-C基因通过激活NF-kB信号通路影响下游ATM,进而增加放疗敏感性。

Objective To investigate the effect of interfering with the expression of VEGF-C on the radiosensitivity of the nasopharyngeal carcinoma CNE-2 cell transplanted tumor and related signaling pathways.Methods The mice with transplanted tumors of nasopharyngeal carcinoma CNE-2 were randomly divided into tumor body group,siRNA-NC group,siRNA-VEGF-C group;irradiation group;siRNA-NC+irradiation group;siRNA-VEGF-C+irradiation group.Tumor growth was observed,and the protein expression of VEGF-C,ATM and P65 were detected by immunohistochemistry.Results Interference of VEGF-C expression in nasopharyngeal carcinoma CNE-2 cell transplanted tumors showed significantly slower tumor growth after irradiation.Immunohistochemical test results showed that after tumor transplantation of mice interfered with VEGF-C,VEGFC and ATM were significantly decreased(P<0.05),while P65 was increased(P<0.05).After combined irradiation,VEGF-C and ATM were significantly decreased(P<0.05).Conclusion In mouse nasopharyngeal carcinoma CNE-2 cell transplantation tumors,interfering with VEGF-C gene affects downstream ATM by activating NF-kB signaling pathway,thereby increasing the sensitivity of radiotherapy.