摘要
活性氧等自由基会引发各种疾病,因此清除自由基或抑制自由基的产生,即抗氧化研究尤为重要。体外抗氧化实验是评价抗氧化活性的重要手段,对抗氧化剂的筛选和开发具有指导意义。根据反应机理对各种体外抗氧化活性评价方法进行了分类,并对常用的评价方法(化学分析法、细胞抗氧化活性法)的反应机理研究进展进行了综述,并提出多种评价方法的联用有助于更准确地评价抗氧化活性。
Free radicals such as reactive oxygen species can cause various diseases,so it is particularly important to scavenge free radicals or inhibit the generation of free radicals,that is,to perform antioxidant research.In vitro antioxidant experiment is an important means to evaluate the antioxidant activity,which has guiding significance for the screening and development of antioxidants.In this paper,we classify various evaluation methods for antioxidant activity in vitro according to the reaction mechanism,and review the research progress in reaction mechanism of common evaluation methods(chemical analysis method and cellular antioxidant activity method).Moreover,we put forward the combination of multiple evaluation methods which can help to evaluate antioxidant activity more accurately.
作者
曾文俊
吕彩梦
丁建宝
李艳萍
马建龙
杨锐
杨晋
ZENG Wenjun;LV Caimeng;DING Jianbao;LI Yanping;MA Jianlong;YANG Rui;YANG Jin(School of Chemistry and Chemical Engineering,North Minzu University,Yinchuan 750021,China;Key Laboratory of Chemical Engineering and Technology,State Ethnic Affairs Commission,Yinchuan 750021,China;Ningxia Wuxing Science and Technology Co.,Ltd.,Yinchuan 750001,China;Ningxia Research Center for Natural Medicine Engineering and Technology,Yinchuan 750021,China;College of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021,China)
出处
《化学与生物工程》
CAS
2022年第12期13-19,共7页
Chemistry & Bioengineering
基金
宁夏回族自治区重点研发计划对外合作专项(2020BFG02016,2019BFH02005)。
关键词
抗氧化活性
评价方法
化学分析法
细胞抗氧化活性
反应机理
antioxidant activity
evaluation method
chemical analysis method
cellular antioxidant activity
reaction mechanism
