杜鹃‘胭脂蜜’组织培养与快速繁殖体系

Tissue culture and rapid propagation system of Rhododendron obtusum‘Yanzhimi’

以杜鹃‘胭脂蜜’(Rhododendron obtusum‘Yanzhimi’)的新生茎段为外植体,以WPM为基本培养基,研究不同生长调节物质对杜鹃组织培养过程中芽诱导、增殖壮苗及生根的影响.结果表明,启动培养中诱导腋芽的最佳培养基为WPM+0.1 mg·L^(-1) IBA+1 mg·L^(-1) ZT,腋芽平均诱导率为43.67%,增殖系数为1.18;采用NAA+TDZ与NAA+ZT的组合分别进行增殖壮苗培养,两种在生长及诱导情况上均呈现不同状态.在添加0.5 mg·L^(-1) NAA和0.1 mg·L^(-1) IBA的1/2WPM基本培养基上,杜鹃的生根效果最佳,生根率达82.5%.该研究为杜鹃优质种苗的繁殖与生产,杜鹃遗传转化体系的建立以及转基因育种奠定了一定基础.

Taking the new stems of Rhododendron obtusum‘Yanzhimi’ as explants and WPM as the basic medium,the effects of different growth regulators on bud induction,proliferation and rooting of Rhododendron tissue culture were studied.The results showed that the best medium for inducing axillary buds in the start-up culture was WPM+0.1 mg·L^(-1) IBA+1 mg·L^(-1) ZT,with the average induction rate of axillary buds of 43.67%and the proliferation coefficient of 1.18.The combination of NAA+TDZ and NAA+ZT was used for the proliferation and strong seedling culture,respectively,and the two types exhibited different states in terms of growth and induction.Rhododendron had the best rooting effect on 1/2 WPM basic medium supplemented with 0.5 mg·L^(-1) NAA and 0.1 mg·L^(-1) IBA,with a rooting rate of 82.5%.This research laid provide references for production of high-quality seedlings,establishment of genetic transformation system and transgenic breeding of Rhododendron.