智能手机辅助铜纳米团簇比率荧光探针检测四环素

Smartphone-assisted ratiometric fluorescence probe based on copper nanoclusters for tetracycline detection

稀土离子Eu^(3+)结合EW-Cu NCs设计双发射荧光探针,由于四环素(Tc)与EW-Cu NCs之间的内滤效应,EW-Cu NCs的荧光被猝灭,同时Tc与Eu^(3+)之间的天线效应(AEE)使得Eu^(3+)的荧光得到增强,基于此,通过探针的荧光双响应模式对Tc进行定量检测,在0.25~72.50μmol/L浓度范围内,对Tc的响应有良好的线性关系,检测限为80 nmol/L.同时根据检测过程中溶液荧光颜色的变化(由绿到红),利用智能手机进行比色法分析,颜色信号比值R/B与Tc浓度在2.5~45.0μmol/L范围内有良好的线性关系.该方法成功用于实际样品牛奶中Tc的检测,并获得满意的回收率,表明EW-Cu NCs具有较高的实际应用价值.

A double-emission fluorescent probe was designed by combining rare earth ion Eu^(3+)and EW-Cu NCs.The fluorescence of EW-Cu NCs was quenched by the internal filtration effect between tetracycline(Tc)and EW-Cu NCs.And the antenna effect between Tc and Eu^(3+)enhanced the fluorescence of Eu^(3+).Based on this,the fluorescence dual response mode of the probe was used to quantitatively detect Tc.The response to Tc showed a good linear relationship in the concentration range of 0.25-72.50μmol/L and the detection limit was 80 nmol/L.At the same time,according to the change of fluorescence color(from green to red)during the detection process,smartphone was used for colorimetric analysis,the color signal ratio R/B and Tc concentration had a good linear relationship in the range of 2.5-45.0μmol/L.This method has been successfully applied to the determination of Tc in milk samples with satisfactory recovery,which indicates that EW-Cu NCs has high practical application value.