微波联合金属离子对麦胚中肽的富集研究

Study on the Enrichment of Peptides in Wheat Germ by Microwave Combined with Metal Ions

为高效富集麦胚多肽,采用微波联合金属离子处理麦胚,考察微波功率、微波时间、ZnSO_(4)浓度、MnSO_(4)浓度、CaCl_(2)浓度对麦胚肽含量和蛋白酶活力的影响,在单因素实验的基础上通过正交试验对工艺参数进行了优化。结果表明:在微波功率400 W,微波时间10 s,ZnSO_(4)浓度1.0 mmol/L,MnSO_(4)浓度1.6 mmol/L,CaCl_(2)浓度1.4 mmol/L的条件下,麦胚中的多肽含量可达228.85 mg/g,蛋白酶活力提高至2268.40 U/g。微波联合金属离子处理对麦胚中肽的富集有显著促进作用。

In order to efficiently enrich wheat germ polypeptide,microwave combined with metal ions were used to treat wheat germ,and the effects of microwave power,microwave time,ZnSO_(4)concentration,MnSO_(4)concentration,and CaCl_(2)concentration on wheat germ peptide content and protease activity were investigated.The process parameters were optimized by orthogonal experiment on the basis of single factor.The results showed that under the conditions of microwave power 400 W,microwave time 10 s,ZnSO_(4)concentration 1.0 mmol/L,MnSO_(4)concentration 1.6 mmol/L and CaCl_(2)concentration 1.4 mmol/L,the polypeptide content in wheat germ could reach 228.85 mg/g,and the protease activity increased to 2268.40 U/g.Microwave combined with metal ions can significantly promote the enrichment of peptides in wheat germ.