在体单向肠灌流法研究胡黄连总苷中4个成分的大鼠肠吸收特性

Study on intestinal absorption characteristics of 4 components of total glucosides in picrorhiza scrophulariiflora by in situ single-pass intestinal perfusion method

[目的]考察胡黄连总苷中胡黄连苷Ⅰ、胡黄连苷Ⅱ、胡黄连苷Ⅳ、草夹竹桃苷共4个成分在大鼠体内不同肠段的吸收情况,阐明胡黄连总苷中4个成分的肠吸收特性。[方法]采用在体单向肠灌流法,运用高效液相色谱(HPLC)技术测定胡黄连总苷肠灌流液中4个成分的浓度,以吸收速率常数(Ka)和有效渗透系数(Peff)为评价指标,考察胡黄连总苷中4个成分分别在十二指肠、空肠、回肠、结肠的吸收特性。[结果]胡黄连总苷中4个成分在大鼠不同肠段均Ka>1.65×10^(-2)/min,Peff>2.0×10^(-5)cm/min,表明这4个成分在不同肠段的吸收速度及吸收能力均良好,但各成分在不同肠段的吸收速度及吸收能力存在一定差异。胡黄连苷Ⅰ在不同肠段的Ka、Peff值差异无统计学意义(P>0.05),胡黄连苷Ⅰ无主要吸收部位;胡黄连苷Ⅱ、胡黄连苷Ⅳ和草夹竹桃苷在不同肠段的Ka、Peff值差异均有统计学意义(P<0.05),且胡黄连苷Ⅱ、胡黄连苷Ⅳ和草夹竹桃苷主要吸收部位分别为十二指肠、空肠和结肠。此外,草夹竹桃苷与其他3个成分比较,Ka、Peff值差异均有统计学意义(P<0.05),且其在不同肠段中吸收均最佳。[结论]本研究阐明了胡黄连总苷中4个成分在不同肠段中吸收特性,为胡黄连总苷的临床合理用药提供参考。

[Objective]To investigate the absorption characteristics of picrosideⅠ,picrosideⅡ,picrosideⅣand androsin in different intestinal segments of rats,and explore the intestinal absorption characteristics of four components of total glucosides in Picrorhiza scrophulariiflora(TGPS).[Methods]The in situ single pass intestinal perfusion method was used,and an HPLC system was utilized to determine the contents of four components of TGPS.The absorption characteristics of four components of TGPS in duodenum,jejunum,ileum and colon were investigated with the absorption rate constant(Ka)and the effective permeability coefficient(Peff)as evaluation indexes.[Results]The Ka and Peff of four components from TGPS in different intestinal segments of rats were respectively all greater than 1.65×10^(-2)/min,2.0×10^(-5) cm/min,indicating that the absorption rate and absorption capacity of the four components in different intestinal segments were good,but there were some differences in the absorption rate and absorption capacity of each component in different intestinal segments.There was no notable difference in in Ka and Peff of picrosideⅠin the different intestinal segments(P>0.05),suggesting that there was no main absorption site of picrosideⅠ;the best absorption sites for picrosideⅡ,picrosideⅣand androsin were the duodenum,jejunum and colon,respectively,and the differences between different intestinal segments were statistically significant(P<0.05).In addition,compared with the other three components,there were significant differences in Ka and Peff of androsin in different intestinal segments(P<0.05),and the absorption of androsin in different intestinal segments was the best.[Conclusion]The study investigated the absorption characteristics of four components of TGPS in different intestinal segment,so as to provide reference for the clinical rational use of TGPS.