Expression of PANDA, LincRNA-p21, PUMA in lung tissues of lung cancer patients in the Xuanwei and non-Xuanwei areas of Yunnan Province

Aim:To study the expression of PANDA,LincRNA-p21,and PUMA in lung tissue of patients with lung cancer from Xuanwei of Yunnan Province.Methods:Forty-five cases of lung cancer patients from Xuanwei and 42 lung cancer cases from non-Xuanwei were enrolled.Extraction of RNA was done using the Trizol kit.Real-time fluorescence quantitative PCR assay was done to obtain the relative expression.Results:Expressions of PANDA,LincRNA-p21,and PUMA in male and female patients or in squamous cell carcinoma and adenocarcinoma were not significantly different(P>0.05).However,expression of LincRNA-p21 in Xuanwei patients was higher than non-Xuanwei patients(P<0.05).Expression of PUMA in tumor tissue was lower than that in normal lung tissue(P<0.05),and i n Xuanwei patients was lower than non-Xuanwei patients(P<0.05).In patients from non-Xuanwei regions,expression of LincRNA-p21 in patients with smoking index>400 was higher than in those<400 and non-smokers.Conclusion:Expressions of PANDA,LincRNA-p21,and PUMA in lung tissues have no gender differences or tissue specificity.High expression of LincRNA-p21 in Xuanwei patients may have relationship with cell damage caused by coal burning pollution in Xuanwei.