甘蔗叶乙酸乙酯部位化学成分研究

Chemical constituents from ethyl acetate extract in Saccharum officinarum leaves

甘蔗是糖类加工产业主要的经济作物,甘蔗叶作为广西特色瑶药,在广西民间及瑶族地区具有悠久的药用历史。该课题组前期发现甘蔗叶乙酸乙酯部位具有抗肿瘤活性,为进一步明确其乙酸乙酯部位的化学成分,该文采用硅胶柱色谱、Sephadex LH-20柱色谱、制备型高效液相色谱等多种分离纯化的方法对甘蔗叶乙酸乙酯部位进行研究。结果表明:从甘蔗叶乙酸乙酯萃取部位分离且鉴定了20个化合物,分别为原儿茶醛(1)、3,4-二羟基-苯甲酸甲酯(2)、3,4-二羟基苯甲酸(3)、3-羟基-4-甲氧基苯甲酸(4)、对羟基苯甲酸(5)、对羟基苯甲醛(6)、对羟基肉桂酸(7)、丁香酸(8)、3,5-二甲氧基对苯二酚(9)、1-hydroxy-benzoyl-4-O-α-L-rhamnopyranoside(10)、对羟基苯甲酸-β-D-吡喃葡萄糖酯苷(11)、槲皮素(12)、小麦黄素(13)、异柽柳素(14)、异鼠李素(15)、5,3′,4′-三羟基-7-甲氧基二氢黄酮(16)、7-O-甲基圣草酚(17)、[(E)-4-(1 S,3 R,4 R)-1-hydroxy-4,5,5-trimethyl-7-oxabicyclo[4.1.0]heptan-1-yl]but-1-en-3-o-ne(18)、blumenol A(19)和胸腺嘧啶脱氧核苷(20)。其中,化合物1-4、6、9-11、13-16、18和20均为首次从甘蔗叶中分离得到。该研究结果为日后甘蔗叶乙酸乙酯部分的进一步开发提供了依据.

Saccharum officinarum is the main cash crop in sugar processing industry,and its leaves are characteristic Yao medicine in Guangxi with a long history.Our recent study showed that its ethyl acetate extract was partly responsible for its in vitro anti-tumor activity.In order to clarify the chemical constituents of this part,the modern separation and purification techniques,such as silica gel column chromatography,Sephadex LH-20 column chromatography,and semi-preparative high-performance liquid chromatography were used to identify the structures of the isolates by their physicochemical properties and modern spectral analysis.The results were as follows:Twenty compounds were all isolated and identified as 3,4-dihydroxybenzaldehyde(1),methyl 3,4-dihydroxy-benzoate(2),3,4-dihydroxy-benzoic acid(3),3-hydroxy-4-methoxybenzoic acid(4),p-hydroxy-benzoic acid(5),p-hydroxybenzyl aldehyde(6),p-hydroxy-cinnamic acid(7),syringic acid(8),3,5-dihydroxy-hydroquinone(9),1-hydroxy-benzoyl-4-O-α-L-rhamnopyranoside(10),p-hydroxy-benzoyl-β-D-glucopyranoside(11),quercetin(12),tricin(13),tamarixetin(14),isorhamnetin(15),5,3′,4′-trihydroxy-7-methoxy-flavanone(16),7-O-Methyleriodictyol(17),[(E)-4-(1 S,3 R,4 R)-1-hydroxy-4,5,5-trimethyl-7-oxabicyclo[4.1.0]heptan-1-yl]but-1-en-3-o-ne(18),blumenol A(19)and thymidine(20),respectively.Compounds 1-4,6,9-11,13-16,18 and 20 were isolated and identified from this plant for the first time.The results provide some basis for its further development.