基于网络药理学的仙茅-淫羊藿药对治疗卵巢早衰机制研究

The Effects of Xianmao-Yinyanghuo Couplet Medicines on Premature Ovarian Failure Based on Network Pharmacology

目的:研究仙茅-淫羊藿药对治疗卵巢早衰(premature ovarian failure,POF)的主要活性成分及其靶点的作用机制。方法:检索人类基因组注释数据库GeneCards,Drugbank数据库获取POF靶点,借助中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)获得仙茅、淫羊藿的潜在活性成分及其对应靶点,使用Bioinformatics&Evolutionary Genomics平台筛选仙茅-淫羊藿药对的活性成分与POF的共有靶点,利用Cytoscape软件构建网络图;采用String数据库构建蛋白质相互作用网络;使用DAVID数据库进行基因本体论富集分析(gene ontology,GO)与KEGG(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。结果:通过筛选得到30个药物活性成分,共涉及靶点278个,与疾病靶点有关的活性成分26个,得到仙茅、淫羊藿药对-疾病共同靶点41个,主要富集于139个生物过程和60条信号通路,涉及PI3K-Akt、甲状腺激素、MAPK等信号通路。结论:仙茅-淫羊藿药对活性靶点主要通过PI3K-Akt、甲状腺激素、MAPK等信号通路调控卵巢细胞过程及代谢发挥治疗作用。

Objective:To study the principal active components and the targets of Xianmao(Curculiginis Rhizoma)-Yinyanghuo(Epimedii Folium)couplet medicines in the treatment of POF.Methods:POF targets were obtained by searching GeneCards and Drugbank databases,the potential active ingredients and its corresponding targets of Xianmao and Yinyanghuo were gained via TCMSP,Bioinformatics&Evolutionary Genomics platforms were used to screen active ingredients of the couplet medicines and its common targets with POF,Cytoscape software was used to construct network planning;String database was adopted to build PPI;DAVID database was used to conduct GO enrichment and KEGG pathways enrichment analysis.Results:Through screening,there were 30 active ingredients,involving 278 targets,26 active ingredients related to disease targets,as well as 41 common targets of Xianmao-Yinyanghuo couplet medicines and disease,primarily enriched in 139 biological processes and 60 signaling pathways,referring to PI3K-Akt signaling pathway,thyroid hormone signaling pathway,MAPK signaling pathway and others.Conclusion:Active targets of Xianmao-Yinyanghuo couplet medicines develop therapeutic effects chiefly through regulating ovary cells and metabolism via PI3K-Akt signaling pathway,thyroid hormone signaling pathway,MAPK signaling pathway and others.