澳洲坚果MibZIP1基因克隆及表达规律分析

Cloning and Expression Analysis of MibZIP1 from Macadamia integrifolia

为研究澳洲坚果(Macadamia integrifolia)中bZIP转录因子家族成员在逆境抗性中潜在功能,从‘桂热1号’品种果实中克隆MibZIP1基因。结合转录因子结构分析和不同处理下表达规律进行分析发现MibZIP1基因序列长度1157 bp,基因编码区长度927 bp,编码308个氨基酸。MibZIP1存在典型bZIP superfamily结构域。系统进化分析鉴定出澳洲坚果MibZIP1、蒂罗花(Telopea speciosissima)TsbZIP60和荷花(Nelumbo nucifera)NnbZIP60亲缘关系较近。组织表达分析结果表明MibZIP1在‘桂热1号’品种叶片中的表达量最低,在‘695’品种枝条中的表达量最高。其在受玉米素、水杨酸、乙烯利和脱落酸处理的“桂热1号”叶片中显著上调表达,在赤霉素和过氧化氢处理的“桂热1号”叶片中表达差异不显著。推测MibZIP1与澳洲坚果抗逆性有关,为研发澳洲坚果新型栽培和激素调控技术提供理论指导。

In order to clarify the potential functions of bZIP transcription factor family members in Macadamia integrifolia under stress response,MibZIP1 was cloned from’Guire 1’fruit.Combined with the analysis of transcription factor structure and expression patterns under different treatments,it was found that the full length of MibZIP1 was 1157 bp,and the ORF was 927 bp,and encoded 308 aa,and had a typical bZIP Superfamily domain.Phylogenetic analysis showed that MibZIP1,TsbZIP60 and NnbZIP60 were closely related.The results of tissue expression analysis showed that MibZIP1 expression was the lowest in leaves of’Guire 1’and the highest in branches of’695’.The expression of MibZIP1 was significantly up-regulated in’Guire 1’leaves treated by zeatin,salicylic acid,ethephon and abscisic acid respectively,but was unchanged treated by gibberellin and hydrogen peroxide respectively.It was speculated that MibZIP1 was related to the stress tolerance of M.integrifolia,which provided theoretical guidance for the development of new cultivation and hormone regulation techniques.