摘要
【目的】研究脂多糖(LPS)诱导猪空肠上皮细胞(IPEC-J2)来建立氧化及炎症模型,分析豆腐果苷(HEL)抗炎及抗氧化的作用机理。【方法】试验分为5组:对照组,LPS组,HEL(25、50和100μM)+LPS组。【结果】HEL会显著降低炎性因子TNF-α、IL-1β和IL-6在mRNA水平的表达;LPS会显著提高氧化相关蛋白(Nrf2和HO-1)的表达和抗氧化酶(SOD和CAT)的活性,显著减少氧化产物(MDA)的含量,HEL表现出相反的结果,显著降低了Nrf2和HO-1的表达量和SOD和CAT的活性,显著增加了MDA的含量。【结论】HEL会缓解LPS所引起的IPEC-J2细胞的氧化及炎性损伤,一定程度上保护细胞免受伤害。
【Objective】The aim of this study is to establish an oxidative and inflammatory model of porcine jejunal epithelial cells(IPEC-J2)induced by lipopolysaccharide(LPS),and explore the anti-inflammatory and anti-oxidation mechanism of helicid(HEL).【Methods】The experiment was divided into 5 groups:control group,LPS group,HEL(25,50 and 100μM)+LPS group.【Results】The results showed that HEL could significantly reduce the expression of TNF-α,IL-1βand IL-6 at mRNA level,and we further verified the anti-inflammatory mechanism of HEL at protein level by ELISA.In terms of oxidative damage,LPS could significantly improve the expression of oxidation related proteins(Nrf2 and HO-1)and the activity of antioxidant enzymes(SOD and CAT),and significantly reduce the content of oxidation products(MDA).Importantly,HEL showed the opposite results,reducing the expression of Nrf2,HO-1 and the activity of SOD and CAT significantly and also significantly increasing the content of MDA.【Conclusion】In conclusion,all the results show that HEL can alleviate the oxidative and inflammatory damage of IPEC-J2 cells caused by LPS,and protect cells from injury to a certain extent.
作者
石乐天
陈勇臻
郭腾达
刘金铄
张光辉
陈鹏举
SHI Letian;CHEN Yongzhen;GUO Tengda;LIU Jinshuo;ZHANG Guanghui;CHEN Pengju(College of Veterinary Medicine,Henan Agricultural University,ZhengZhou,450002;Jiaozuo Institute of Technology,JiaoZuo,454000;Henan Institute of Modern Chinese Veterinary Medicine,ZhengZhou,450002;Shandong Xindehui Biotechnology Co.,Ltd,Yuncheng,Shandong Province 274700,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2022年第10期2595-2600,I0001-I0005,共11页
Xinjiang Agricultural Sciences
基金
国家重点研发计划“畜禽重大疫病防控与高效安全养殖综合技术研发”重点专项“新型动物药剂创制与产业化”(2017YFD0501400)。
