补喂竹叶提取物对伊犁马挤奶期间心率变异性的影响

Effects of Bamboo Leaf Extract on Heart Rate Variability of Yili Horses during Milking

【目的】研究补喂竹叶提取物后伊犁马挤奶期间心率变异性的变化,分析不同剂量和不同饲喂试验期对伊犁马挤奶应激的影响。【方法】选取24匹伊犁母马,其体型结构相似,胎次相近,进行为期60 d的补饲竹叶提取物,0~20 d为第1阶段,21~40 d为第2阶段,41~60 d为第3阶段。采集马匹挤奶期间心率变异性指标。将24匹伊犁马随机分为对照组0 g/d;试验Ⅰ组10 g/d;试验Ⅱ组20 g/d;试验Ⅲ组30 g/d四组,分析不同时期和剂量的HRV差异性。【结果】第3阶段SDT HR显著高于第1阶段(P<0.05);第2和第3阶段RMSSD均显著高于第1阶段(P<0.05);第3阶段SD 1显著高于第1阶段(P<0.05);第3阶段SD 2极显著高于第2阶段(P<0.01),且第2阶段又极显著高于第1阶段(P<0.01)。第3阶段VLF极显著高于第1和第2阶段(P<0.01);第3阶段的LF和HF两个指标均极显著高于第1阶段和第2阶段(P<0.01);第3阶段TP极显著高于第1阶段和第2阶段(P<0.01)。对照组Mean HR显著高于试验Ⅰ组和试验Ⅲ组(P<0.05);第Ⅱ组SD 2极显著高于第Ⅳ组(P<0.01),且显著高于对照组(P<0.05);对照组SD 2和VLF显著低于试验Ⅰ组(P<0.05);对照组和试验Ⅰ组LF显著高于试验Ⅲ组(P<0.05);试验Ⅰ组中HF、TP显著高于试验Ⅲ组(P<0.05);对照组LF/HF显著低于试验Ⅰ组(P>0.05)。【结论】伊犁马补喂竹叶提取物能有效降低马匹交感神经活动,减少其挤奶时的应激反应。

【Objective】To study the changes in heart rate variability(HRV)of Yili horses during milking after supplementary feeding with bamboo leaf extract,and explore the effects of different doses and different feeding periods on milking stress of Yili horses.【Methods】In this experiment,24 Yili mares with similar body shape and structure and similar parity were selected and they were fed with bamboo leaf extract for 60 days.0-20 days was taken as stage 1,21-40 days was taken as stage 2,and 41-60 days was taken as stage 3.The indexes of HRV were collected during milking.The selected Yili horses were randomly divided into control group(0 g/d),groupⅠ(10 g/d),groupⅡ(20 g/d)and groupⅢ(30 g/d).HRV differences in different periods and doses were analyzed.【Results】The results during different feeding test periods showed that SDT HR in stage 3 was significantly higher than that in stage 1(P<0.05);RMSSD in stage 2 and 3 were significantly higher than that in stage 1(P<0.05);SD 1 in stage 3 was significantly higher than that in stage 1(P<0.05);SD 2 in stage 3 was significantly higher than that in stage 2(P<0.01),and stage 2 was significantly higher than that in stage 1(P<0.01).VLF in stage 3 was significantly higher than that in stage 1 and 2(P<0.01);LF and HF in stage 3 were significantly higher than those in stage 1 and 2(P<0.01);TP in stage 3 was significantly higher than that in stage 1 and 2(P<0.01).The analysis results in different groups showed that the Mean HR in control group was significantly higher than that in test group I and the test group III(P<0.05);SD 2 in groupⅡwas significantly higher than that in groupⅣ(P<0.01)and significantly higher than that in control group(P<0.05);SD 2 and VLF in control group were significantly lower than those in test group I(P<0.05);LF in control group and test groupⅠwas significantly higher than that in test groupⅢ(P<0.05);HF and TP in groupⅠwere significantly higher than those in groupⅢ(P<0.05);LF/HF in control group was significantly lower than that in test group I(P>0.05).【Conclusion】Considering the HRV changes of horses,supplementary feeding with bamboo leaf extract to Yili horses can effectively reduce the sympathetic nerve activity and stress response of horses during milking under the test conditions.