InDel标记在萝卜杂交种圆都1号纯度鉴定中的应用

Application of InDel marker in purity identification of radish hybrid Yuandu No.1

为准确高效鉴定萝卜杂交种的种子纯度,在萝卜杂交种圆都1号亲本全基因组重测序的基础上,利用亲本间的差异性位点设计特异插入/缺失引物,通过InDel标记开展高效准确的圆都1号杂交种纯度鉴定。试验选定引物L-4,其亲本带型互补、清晰度高、分离速度快,以此对358株萝卜杂交种圆都1号进行纯度鉴定,检测出母本或异花粉杂株5株,纯度为98.60%。InDel标记的分子检测结果与田间表型鉴定结果对比显示有355株萝卜的鉴定结果完全一致,吻合度高达99.16%。因此,应用InDel标记引物L-4能够对萝卜杂交种圆都1号进行高效准确的纯度鉴定。

Accurate and efficient identification of hybrid purity is of great significance for the seed propagation and extention of radish hybrid.The insertion/deletion(InDel)primer L-4 is designed to identify the purity of Yuandu No.1 based on the genome resequencing and different Insertion/Deletion locus of the parents of Yuandou No.1.The primer L-4 was selected in the experiment.Its parental bands were complementary,with high clarity and fast separation speed.Based on this primer,358 radish hybrids Yuandu No.1 were identified for purity.Five female parents or heteropollens were detected,with a purity of 98.60%.The results of molecular detection and field phenotypic identification showed that 355 radish plants have the same identification results,with 99.16%consistency.Therefore,the indel primer L-4 can be used to efficiently and accurately identify the purity of radish hybrid Yuandu No.1.