基于Illumina Hiseq高通量测序红曲霉转录组学分析

Transcriptome analysis of Monascus based on Illumina Hiseq high-throughput sequencing

分别以铵态氮、亚硝态氮为氮源培养安卡红曲霉(Monascus anka)GZUM-25,采用Illumina二代测序平台(Illumina Hiseq)对红曲霉GZUM-25进行转录组测序分析,分析不同氮源条件下调控红曲霉菌丝生长和产色素的差异表达基因,并对基因进行GO功能富集及KEGG通路富集分析。结果表明,从铵态氮和亚硝态氮培养下的安卡红曲霉GZUM-25基因组中共获得95个差异表达基因,其中,上调基因有37个,下调基因有58个。差异表达基因主要富集的功能为生物学过程、细胞组分和分子功能,富集的通路为磷酸肌醇代谢、淀粉和蔗糖代谢、苯乙烯降解、氨基苯基酸酯降解。

Using ammonium nitrogen and nitrite nitrogen as the nitrogen sources to cultivate Monascus anka GZUM-25, the transcriptome sequencing analysis of M. anka GZUM-25 was conducted by Illumina Hiseq, the differentially expressed genes regulating mycelial growth and pigment production of Monascus under different nitrogen sources conditions were analyzed, and the genes were studied by GO functional enrichment and KEGG pathway enrichment analysis. The results showed that a total of 95 differentially expressed genes were obtained from M. anka GZUM-25 under ammonium nitrogen and nitrite nitrogen cultivation, of which 37 were up-regulated genes and 58 were down-regulated genes. The main enrichment functions of differentially expressed genes were biological processes, cellular components and molecular functions. The enrichment pathways were inositol phosphate metabolism, starch and sucrose metabolism, styrene degradation, and aminophenyl ester degradation.