低敏宠物核心料对食源性过敏小鼠的作用效果评价

Evaluation of the Effect of Hypoallergenic Pet Core Material on Food-borne Allergic Mice

本试验旨在研究低敏宠物核心料的抗过敏效果并初步探讨其可能机制。试验以5周龄雌性BALB/c小鼠为实验动物,通过OVA诱导食源性过敏小鼠模型。同时给予低敏宠物核心料,检测其对食源性过敏小鼠肠道组织结构以及过敏反应的影响,并通过检测小鼠免疫球蛋白、细胞因子、组胺水平,脾、胸腺器官指数,初步探讨其抗过敏的可能机理。结果显示:与对照组相比,食源性过敏小鼠体重增长缓慢,平均日增重极显著降低(P<0.01),绒毛高度、隐窝深度、二者比值(V/C)均显著降低(P<0.05或P<0.01),腹泻率极显著升高(P<0.01),达到86%,直肠温度极显著降低(P<0.01),过敏反应评分极显著增加(P<0.01),血清IgE、IgG、His、IL-4水平均极显著上升(P<0.01),IL-10水平极显著下降(P<0.01),肥大细胞脱颗粒比率显著增加(P<0.05),胸腺及脾指数则均极显著降低(P<0.01);给予低敏宠物核心料的小鼠上述指标较过敏小鼠均显著改善。本试验采用的低敏宠物核心料可通过抑制肥大细胞脱颗粒,减少组织胺的释放,抑制OVA诱导的食源性小鼠过敏反应的发生,减轻过敏反应症状,同时可改善过敏小鼠肠道组织结构,促进营养物质的吸收。

The purpose of this experiment was to study the anti-allergic effect of hypoallergenic pet core material and preliminarily explore its possible mechanism.Five weeks old female BALB/c mice were used in the experimental.Ovalbumin(OVA)was administered to establish a food-borne allergy mouse model.Meanwhile,hypoallergenic pet core material was given to investigate the effect of this core material on the intestinal structure and allergic reaction of food-borne allergic mice.The anti-allergic mechanism was explored through the detection of immunoglobulin,cytokines,histamine,and immune organ index in mice.Compared with the control group,the average daily gain of food-induced allergic mice was significantly decreased(P<0.01);the villus height,crypt depth,and the ratio of which were significantly decreased(P<0.05 or P<0.01);diarrhea rate was up to 86%which was highly significantly increased(P<0.01),the rectal temperature was significantly decreased(P<0.01),and the allergic reaction score was significantly increased(P<0.01);the serum levels of IgE,IgG,His,and IL-4 were extremely significantly increased(P<0.01),the IL-10 levels were significantly decreased(P<0.01),and the number of degranulated mast cells was significantly increased(P<0.05);thymus and spleen index were significantly decreased(P<0.01);the above indexes of mice given hypoallergenic pet core material were significantly improved compared with the model mice.The hypoallergenic pet core material can inhibit the degranulation of mast cells,reduce the release of histamine,inhibit the occurrence of food induced allergic reaction in mice induced by OVA,reduce the symptoms of allergic reaction,increase the height of villi,improve the intestinal tissue structure of allergic mice and the absorption of nutrients of mice.