短时受精对夫精IVF胚胎参数及妊娠结局的影响

Effects of short-term fertilization on IVF embryo parameters and pregnancy outcomes

目的 探究短时受精对夫精体外受精(IVF)胚胎参数及妊娠结局的影响。方法 725例进行IVF的不孕患者,根据IVF方式分为短时组(347例)和常规组(378例)。常规组采用常规长时受精,短时组采用短时受精。比较两组胚胎参数及妊娠结局(平均获卵数、受精率、正常受精率、多原核受精率、卵裂率、优质胚胎率、平均移植胚胎数、冷冻周期率、平均冷冻胚胎数、种植率、临床妊娠率)。结果 短时组的优质胚胎率48.99%高于常规组的37.30%,差异具有统计学意义(P<0.05)、短时组平均获卵数(13.63±6.84)枚、受精率77.23%、正常受精率62.25%、多原核受精率6.05%、卵裂率97.12%、平均移植胚胎数(1.71±0.86)枚、冷冻周期率78.96%、平均冷冻胚胎数(3.23±2.54)枚、种植率46.40%、临床妊娠率63.40%与常规组的(13.38±6.88)枚、77.25%、62.96%、7.67%、97.35%、(1.70±0.88)枚、80.69%、(3.19±2.55)枚、43.39%、59.79%比较,差异均无统计学意义(P>0.05)。结论 不孕患者在IVF过程中短时受精的优质胚胎率由于缩短精卵孵育时间,在一定程度上能提高胚胎质量,短时受精可以在不影响正常受精率的前提下为临床提供更多的优质胚胎,可在临床推广使用。

Objective To investigate the effects of short-term fertilization on in vitro fertilization(IVF) embryo parameters and pregnancy outcomes. Methods A total of 725 infertile patients treated with IVF were divided into short-term group(347 cases) and conventional group(378 cases) according to the mode of IVF. The conventional group used conventional long-term fertilization, and the short-term group used shortterm fertilization. Both groups were compared in terms of embryo parameters and pregnancy outcomes(average number of eggs retrieved, fertilization rate, normal fertilization rate, polypronuclear fertilization rate, cleavage rate, high-quality embryo rate, average number of embryos transferred, freezing cycle rate, average number of frozen embryos, implantation rate, and clinical pregnancy rate). Results The high-quality embryo rate in the short-term group was 48.99%, which was higher than 37.30% in the conventional group, and the difference was statistically significant(P<0.05). The short-term group had average number of eggs retrieved of(13.63±6.84)eggs, fertilization rate of 77.23%, normal fertilization rate of 62.25%, polypronuclear fertilization rate of 6.05%,cleavage rate of 97.01%, average number of embryos transferred of(1.71±0.86) embryos, freezing cycle rate of 78.96%, average number of frozen embryos of(3.23±2.54) embryos, implantation rate of 46.40%, and clinical pregnancy rate of 63.40%, which had no statistically significant difference compared with(13.38±6.88) eggs,77.25%, 62.96%, 7.67%, 97.35%,(1.70±0.88) embryos, 80.69%,(3.19±2.55) embryos, 43.39% and 59.79% in the conventional group(P>0.05). Conclusion For infertile patients with IVF, high-quality embryo rate of shortterm fertilization due to shorten the incubation time of sperm and ovum, short-term fertilization can improve the quality of embryos to a certain extent. Short-term fertilization can provide more high-quality embryos for the clinic without affecting the normal fertilization rate, which can be promoted in clinical practice.