小豆AUX/IAA基因家族全基因组鉴定及表达分析

Genome-wide Identification and Expression Analysis of AUX/IAA Gene Family Members in Vigna angularis

【目的】生长素是一种必需的植物激素,而AUX/IAA是生长素原初响应因子在环境胁迫中植物生长和发育中起着至关重要的作用。为了解VaIAAs在小豆生长发育过程中的生物学功能,对VaIAAs基因家族进行全基因组鉴定和分析。【方法】利用生物信息学对小豆的AUX/IAA进行全基因组鉴定和分析,基于RNA-seq分析VaAUX/IAA基因家族在各组织的表达模式。【结果】在小豆全基因组共鉴定到41个VaIAAs基因,亚细胞定位显示均为定位为细胞核。系统发育分析显示来自小豆、拟南芥(Arabidopsis thaliana)和水稻(Oryza sativa)的AUX/IAA蛋白聚集成6组。基因结构分析表明,所有基因均含有内含子数目1~8个,VaIAAs基因包含1~21个外显子。顺式作用元件分析发现参与响应生物/非生物胁迫顺式作用元件最多,包括最常识别到的ATTATA.bos、G-box和sp1基序,还发现富集在厌氧诱导响应元件ARE和植物抗逆相关的MYB元件。蛋白-蛋白互作网络分析发现,所有VaIAAs均与ARF相连,此外还有部分基因与AXR3(生长素诱导阻遏因子)、MP(转录因子B3家族蛋白)等基因互作。VaIAAs具有组织特异性,在不同组织的基因表达水平差异较大。【结论】小豆AUX/IAA基因家族的基因结构、系统进化、蛋白质-蛋白质网络互作等生物信息学分析结果,将为揭示小豆AUX/IAA基因家族在小豆生长发育过程中的功能提供重要的线索。

【Objective】Auxin is an essential plant hormone,and AUX/IAA is an important auxin initial response factor that plays a crucial role in the growth and development of plants under environmental stress.To understand the biological function of VaIAAs in the growth and development of Vigna angularis,genome-wide of VaIAAs gene family was identified and analyzed.【Method】Bioinformatics was used to identify and analyze genome-wide of AUX/IAA in Vigna angularis.Expression patterns of VaAUX/IAA gene family in various tissues were analyzed based on RNA-seq.【Result】A total of 41 VaIAAs genes were identified in genome-wide of V.angularis,and subcellular localization showed that all of them were localized to the nucleus.Phylogenetic analysis revealed that AUX/IAA proteins from V.angularis,Arabidopsis thaliana,and Oryza sativa clustered into six groups.Gene structure analysis showed that all the genes contained 1-8 introns and VaAUX/IAA genes contained 1-21 exons.Analysis of cis-acting elements found that cis-acting elements involved in response to biological/abiotic stress were the most,including the most commonly recognized ATTATA.bos,G-box and sp1 motifs.The enrichment of anaerobic-induced response element ARE and plant stress resistance-related MYB elements was also found.Protein-protein interaction network analysis showed that all VaIAAs were linked to ARF,in addition,some genes interacting with AXR3(auxin-induced repressor),MP(transcription factor B3 family protein),and etc.VaIAAs were tissue-specific,with large differences in gene expression levels in different tissues.【Conclusion】The results of bioinformatics analysis of gene structure,phylogeny,and protein-protein network interactions of the V.angularis AUX/IAA gene family will provide important clues to reveal the functions of AUX/IAA gene family in the growth and development of V.angularis.