血清lncRNA-MALAT-1、miR-143的表达水平与急性髓系白血病临床特征和预后的关系

Relationship between the expression levels of serum lncRNA-MALAT-1 and miR-143 and the clinical features and prognosis of acute myeloid leukemia

目的研究血清长链非编码RNA(lncRNA)-肺腺癌转移相关转录本1(MALAT-1)、微RNA-143(miR-143)的表达水平与急性髓系白血病(AML)临床特征和预后的关系。方法选取2017年1月至2019年4月四川省南充市中心医院(以下简称“我院”)血液内科收治的87例AML患者为AML组,另选取32例同期于我院体检健康者为对照组。采用qPCR检测血清lncRNA-MALAT-1、miR-143表达。分析二者表达相关性及与AML临床特征的关系。根据血清lncRNA-MALAT-1、miR-143表达均值分为高、低表达组,采用Kaplan-Meier法绘制AML患者生存曲线。结果AML组血清lncRNA-MALAT-1表达高于对照组,miR-143表达低于对照组(P<0.01)。Pearson相关系数显示,AML组血清lncRNA-MALAT-1与miR-143表达呈负相关(r=-0.709,P<0.01)。不同白细胞计数、骨髓原始细胞比率、预后危险分层的AML患者血清lncRNA-MALAT-1、miR-143表达比较,差异有统计学意义(P<0.05)。lncRNA-MALAT-1高表达组与lncRNA-MALAT-1低表达组生存曲线比较,差异有统计学意义(P<0.05);miR-143高表达组与miR-143低表达组生存曲线比较,差异有统计学意义(P<0.05)。结论AML患者血清lncRNA-MALAT-1高表达和miR-143低表达,与白细胞计数、骨髓原始细胞比率、预后危险分层和预后有关。

Objective To investigate the relationship between the expression levels of serum long-stranded non-coding RNA(lncRNA)-lung adenocarcinoma metastasis-associated transcript 1(MALAT-1)and microRNA-143(miR-143)and the clinical features and prognosis of acute myeloid leukemia.Methods Eighty-seven patients with acute myelogenous leukemia(AML)admitted to the Department of Hematology of Nanchong Central Hospital(hereinafter referred to as“our hospital”)from January 2017 to April 2019 were selected as the AML group,and 32 healthy patients who underwent physical examination in our hospital during the same period were selected as the control group.The expressions of serum lncRNA-MALAT-1 and miR-143 were detected by qPCR.The correlation between the two expressions and the clinical features of AML was analyzed.According to the mean expression values of serum lncRNA-MALAT-1 and miR-143,patients were divided into high and low expression groups.Kaplan-Meier method was used to draw the survival curve of AML patients.Results Serum lncRNA-MALAT-1 expression in the AML group was higher than that in the control group,and miR-143 expression was lower than that in the control group(P<0.01).Pearson’s correlation coefficient showed that serum lncRNA-MALAT-1 was negatively correlated with miR-143 expression in the AML group(r=-0.709,P<0.01).Comparison of serum lncRNA-MALAT-1 and miR-143 expression in AML patients with different white blood cell counts,bone marrow primitive cell ratios,and prognostic risk stratification showed statistically significant differences(P<0.05).Comparison of survival curves between the high expression group and the low expression group of lncRNA-MALAT-1 showed a statistically significant difference (P<0.05);Comparison of survival curves between the high expression group and the low expression group of miRNA showed a statistically significant difference overall survival rate was higher in the miR-143 high expression group than in the miR-143 low expression group (P<0.05). Conclusion High expression of serum lncRNA-MALAT-1 and low expression of miR-143 in AML patients is associated with leukocyte count, bone marrow primitive cell ratio, prognostic risk stratification and prognosis.