麻杏止嗽散对咳嗽变异型哮喘模型小鼠IFN-γ、IL-8水平的影响

Effect of Hemp Cough Powder on IFN-γand IL-8 Levels in Cough Variant Asthma Model

目的通过观察麻杏止嗽散对咳嗽变异型哮喘模型小鼠外周血干扰素γ(interferonγ,IFN-γ)、白细胞介素-8(interleukin8,IL-8)的影响,探讨麻杏止嗽散对咳嗽变异型哮喘(cough variant asthma,CVA)的免疫调节机制,为中药开发提供实验依据。方法采用氢氧化铝和卵蛋白致敏,并运用卵蛋白激发的实验方法复制CVA模型小鼠,随机分为5组,各组予以相应治疗3周后腹主动脉取血,检测血清IFN-γ、IL-8水平。结果麻杏止嗽散治疗CVA可能与改变小鼠血清中IL-8、IFN-γ有关,麻杏止嗽散可明显提高CVA模型小鼠血清IFN-γ的水平,降低血清IL-8的水平。结论麻杏止嗽散能提高IFN-γ水平、抑制IL-8的水平,可明显降低CVA模型小鼠炎症反应程度,具有抗气道炎症及免疫系统调节作用,可能是中药复方药物治疗CVA的机制之一。

Objective To observe the effects on peripheral interferonγ(interferonγ,IFN-γ),and interleukin 8(interleukin8,IL-8),to explore the immune regulation mechanism of this effect on cough variant asthma,and to provide an experimental basis for the development of traditional Chinese medicine.Methods CVA model mice were replicated and randomly divided into 5 groups.Each group was treated for 3 weeks to obtain blood from serum IFN-γand IL-8.Results The treatment of CVA may be related to changing IL-8 and IFN-γin the serum of mice,which can significantly increase the serum levels of IFN-γand reduce IL-8 in CVA model mice.Conclusion By increasing the level of IFN-γ,it can significantly reduce the inflammatory response of IL-8 in CVA model mice,and has anti-airway inflammation and immune system regulation,which may be one of the mechanisms of the compound treatment of CVA with traditional Chinese medicine.