5种晚熟柑橘多甲氧基黄酮提取方法比较及应用

Comparison and Application of Extraction Methods of Polymethoxy Flavonoids from Five Late-Maturing Citrus Pericarp

【目的】建立晚熟柑橘果皮中多甲氧基黄酮(PMFs)快速、高效提取方法,比较5种晚熟柑橘果皮中PMFs的含量差异,筛选出适合晚熟柑橘PMFs提取的最优处理组合,为柑橘多甲氧基黄酮的分离检测及高含量PMFs的晚熟柑橘品种提供参考依据。【方法】以‘黄果柑’为研究材料,用超声提取、振荡提取和微波提取3种方法,分别设置提取剂、时间和料液比三个因素,进行三因素三水平正交实验,从27种组合中筛选出3组最优组合,以得到的3种组合对五种晚熟柑橘PMFs含量进行提取、比较和分析。【结果】‘黄果柑’PMFs在超声法中的最优条件是甲醇超声45 min、料液比1∶20(U2);在振荡法中最高的是甲醇振荡180 min、料液比1∶30(O3);微波法中最优条件是甲醇微波提取45 s、料液比1∶30(M2)。综合考虑时间、提取效率等因素,‘黄果柑’PMFs提取的最优处理为M2。另外,在3种最优处理中,PMFs提取量最高的均为‘沃柑’,‘黄果柑’次之。‘春见’‘大雅’之间PMFS的提取量差异不大,‘塔罗科血橙’的PMFs提取量最小。

【Objective】The purpose of this study is to establish a rapid and efficient method for extracting polymethoxy flavonoids(PMFs)from late-maturing citrus peels. By comparing the content of PMFs in the pericarp of five kinds of late-maturing citrus,the optimal treatment combination of PMFs extraction from late-maturing ripening citrus was selected finally. This provides a reference basis for the separation and detection of PMFs in citrus and the late-maturing citrus varieties with high PMFs content.【Method】This study was made by using ’Huangguogan’ as the research material,and a three-factor and three-level orthogonal test were carried out by using three methods including ultrasonic extraction,oscillation extraction and microwave extraction,and setting three factors of extractant,time and ratio of material to respectively. Three groups of optimal combinations were selected form 27 combinations finally,and the PMFs contents of five kinds of late-maturing citrus were analyzed.【Result】The highest extraction amount in the peel of ’Huangguogan’ was U2 in ultrasonic extraction,O3 in oscillatory extraction and M2 in microwave extraction. Considering the time,extraction efficiency and other factors,the optimal treatment combination for PMFs extraction of ’Huangguogang’ is M2. In addition,in the same treatment method,the highest extraction amount of PMFs is ’Orah mandarin’,followed by ’Huangguogan’. There is little difference in extraction quantity between ’Harumi’ and ’Daya’ orange,and the extraction amount of PMFs from ’Tarocco’ is the smallest.