中药复配物对猕猴桃溃疡病菌抑菌机理研究

Study on the Antibacterial Mechanism of Chinese Medicine Compound on Kiwifruit Ulcer Pathogen

【目的】研究由黄芩、石菖蒲和丁香等中药组成的提取复配物对猕猴桃溃疡病菌的抑菌机理。【方法】通过牛津杯法测定中药复配物对猕猴桃溃疡病菌的最低抑菌浓度和最低杀菌浓度,并对菌体的生长曲线、细胞壁和细胞膜完整性、胞内DNA含量变化测定,结合细菌电镜透射图,综合分析中药复配物对猕猴桃溃疡病菌的作用机理。【结果】测定得到其最低抑菌浓度为3.125 mg/mL;最低杀菌浓度为12.5 mg/mL。中药复配物可破坏猕猴桃溃疡病菌的细胞壁和细胞膜,使胞内物质溢出,DNA含量降低,还可破坏菌体形态结构,使菌体扭曲变形,生命活性降低。【结论】中药复配物对猕猴桃溃疡病菌有较强的抑制效果,其抑菌机理为影响菌体细胞形态、细胞壁、细胞膜和遗传物质等。

【Objective】To study the bacteriostasis mechanism of the compound extracted from Acorus tatarinowii,Scutellaria baicalensis,Syzygium aromaticum and other traditional chinese medicines against the pathogen of kiwifruit ulcer.【Method】The minimum inhibitory concentration and the minimum bactericidal concentration of Chinese herbal compound against kiwifruit ulcer pathogen were determined by Oxford cup method. The growth curve,cell wall and cell membrane integrity,intracellular DNA content changes of the bacteria were measured,combined with the transmission electron microscope of bacteria,the mechanism of action of chinese medicine compound on kiwifruit ulcer pathogen was comprehensively analyzed.【Result】The minimum inhibitory concentration was 3.125 mg/mL. The minimum sterilization concentration is 12.5 mg/mL. Chinese medicine compound can destroy the cell wall and cell membrane of kiwifruit ulcer pathogen,make the intracellular material overflow,reduce the DNA content,but also destroy the morphological structure of the bacteria,make the bacteria distorted,reduce the life activity.【Conclusion】The compound of traditional chinese medicine has strong inhibitory effect on kiwifruit ulcer pathogen,and its antibacterial mechanism is affecting the morphology of cell,cell wall,cell membrane,genetic material and so on.