黄化对牡丹枝条解剖结构及不定根发生的影响

The Effect of Etiolation on Shoots Anatomical Structure and Adventitious Root Formation of Tree Peony

【目的】为提高牡丹压条繁殖效率。【方法】以牡丹品种‘洛阳红’和‘黄金翠’作为试验材料,对当年生枝条进行黄化处理后,进行环剥和IBA(indole-3-butyric acid,吲哚丁酸)处理,探究黄化后牡丹枝条解剖结构的变化、不定根生根率和根系质量。【结果】‘洛阳红’和‘黄金翠’枝条黄化处理后,黄化部位的皮层薄壁细胞增多、木栓层发育延迟、木栓层外组织松散,且最先出现不定根。2个品种枝条黄化处理后的生根率和生根质量均优于对照枝条,一级根生根率分别为88.9%、97.8%,其中‘黄金翠’黄化枝条的一级根平均数量、二级根平均数量、每一级根上二级根平均数量、一级根平均根长以及根直径分别为8.08条、59.6条、6.8条、12.42 cm和1.97 mm,表现最佳。【结论】黄化处理可降低牡丹枝条的木质化程度,缩短压条繁殖时的不定根发生时间,提高不定根生根率和根系质量。

【Objective】In order to improve the reproductive efficiency of tree peony layering.【Method】Tree peony cultivars ’Luoyang Hong’ and ’Huangjin Cui’ were used as experimental materials,after etiolation treatment and combination with stem gridling and IBA(indole-3-butyric acid)treatment,the effects of etiolation treatment on shoot anatomical structure,adventitious root rooting rate and root quality of tree peony were studied. 【Result】In the etiolated sites of ’Luoyang Hong’ and ’Huangjin Cui’,there were more parenchyma cells,delayed development of phellem cells,loose tissue outside phellem,and adventitious roots appeared first compared with the control. The rooting rate and root quality of ’Luoyang Hong’and ’Huangjin Cui’ with etiolation treatment were better than those of the control,the rooting rate of primary root was 88.9% and 97.8%,respectively. The average primary root number,the average secondary root number,the average secondary root number on each primary root,average primary root length and diameter of ’Huangjin Cui’ were 8.08,59.6,6.8,12.42 cm and 1.97 mm,respectively,which was the best performance in all treatments.【Conclusion】The etiolation treatment reduced the lignification degree of tree peony shoots,shorten the time of adventitious root formation during layering propagation,and improved the rooting rate and root quality of adventitious roots.