原核生物转录调控研究技术及进展

Research Technology and Progress in Transcriptional Regulation in Prokaryotes

原核生物基因表达调控主要发生在转录水平,研究原核生物的转录调控有利于了解其基因表达调节机制。近年来,随着分子生物学及相关技术的突破,转录调控研究技术也不断发展,因此主要综述了原核生物转录调控的技术方法及其新进展,包括凝胶电泳迁移率实验、DNase I足迹技术、染色质免疫共沉淀技术、微量热泳动技术、等温滴定量热法及细菌单杂交系统,以期系统地了解这些方法的优缺点和适用性,帮助研究者更好的利用原核生物转录调控为人类造福。

The regulation of gene expression in prokaryotes mainly occurs at the transcriptional level.Knowledge on the regulation of transcription in prokaryotes is conducive to understanding the mechanism of gene expression regulation.In recent years,with the breakthrough of molecular biology and related technologies,the research technology of transcriptional regulation has also been continuously developed.The technical methods and new developments of prokaryotic transcriptional regulation are reviewed in the present study,including electrophoretic mobility shift assay,DNase I footprinting technology,chromatin immunoprecipitation technology,microthermophoresis technology,isothermal titration calorimetry and bacterial one hybrid system,aiming to systematically understand the advantages/disadvantages and suitability of these methods,help researchers to better use the transcriptional regulation of prokaryotes for bringing great benefits to human life.