摘要
目的对孕妇产前血清学检查中抗G抗体鉴定,区分抗G和普通抗D及抗D和抗C混合抗体,采用O型ccDEE及Ccdee两种细胞作为吸附细胞,两步吸收放散方法,区别鉴定孕妇抗G抗体。方法选取较少报道使用的ccDEE及Ccdee两种细胞作为吸附细胞,两步法吸收放散,并通过ABO血型鉴定、Rh分型、直接抗人球蛋白试验、不规则抗体筛查、抗体效价检测等试验鉴定抗G。结果试验设计和方法能有效鉴定区别抗G和抗D及抗C。结论采用O型ccDEE及Ccdee两种细胞作为吸附细胞,两步吸收放散方法,鉴定抗G抗体,细胞来源广泛,节约吸收时间,鉴定结果准确。
Objective Anti-G antibodies in prenatal serological testing were identified to distinguish anti-G from anti-D and both anti-D and anti-C antibodies.O-type ccDEE and Ccdee cells were used as adsorbed cells,and“double adsorptions”was used.Methods The ccDEE and Ccdee cells less reported were selected as adsorbed cells,double adsorptions were performed.ABO blood typing,Rh typing,direct Combs test,irregular antibody screening and antibody titer detection and other tests were used to identify Anti-G.Results The experimental design and method can effectively identify anti-G and distinguish anti-D and anti-C from anti-G.Conclusion O-type ccDEE and Ccdee cells were used as adsorbed cells.The double adsorptions were used to identify Anti-G.The cell source was wide,the absorption time was saved,and the identification result was accurate.
作者
黄靓
刘丹
凤维杰
刘盼盼
HUANG Liang;LIU Dan;FENG Wei-jie(Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030)
出处
《临床输血与检验》
CAS
2022年第6期754-758,共5页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
孕妇产前血清学检查
不规则抗体鉴定
抗-G抗体
吸收放散试验
Prenatal serological examination of pregnant women
Identification of irregular antibodies
Anti-G antibodies
Absorption-elution test